Detailed information of TA system TA218417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 21306..21890
• Replicon: plasmid pXMC5X702-tetX-145k
• Accession: NZ_CP084302
• Organism: Acinetobacter pseudolwoffii strain XMC5X702

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: LDO52_RS15320
• Protein ID: WP_000286964.1
• Coordinates: 21306..21608 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LDO52_RS15325
• Protein ID: WP_000985609.1
• Coordinates: 21609..21890 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO52_RS15285 (LDO52_15285)	17143..17478	-	336	WP_000618091.1	IS66 family insertion sequence element accessory protein TnpB	-
LDO52_RS15290 (LDO52_15290)	17475..17858	-	384	WP_001055589.1	transposase	-
LDO52_RS15295 (LDO52_15295)	17884..18216	+	333	WP_117008139.1	MerR family DNA-binding protein	-
LDO52_RS15300 (LDO52_15300)	18463..18750	+	288	WP_000438825.1	BrnT family toxin	-
LDO52_RS15305 (LDO52_15305)	18737..19051	+	315	WP_004728120.1	BrnA antitoxin family protein	-
LDO52_RS15310 (LDO52_15310)	19140..19670	-	531	WP_000172759.1	hypothetical protein	-
LDO52_RS15315 (LDO52_15315)	19861..21186	+	1326	WP_001280601.1	ISNCY family transposase	-
LDO52_RS15320 (LDO52_15320)	21306..21608	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDO52_RS15325 (LDO52_15325)	21609..21890	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
LDO52_RS15330 (LDO52_15330)	21970..22389	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
LDO52_RS15335 (LDO52_15335)	22529..22849	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LDO52_RS15340 (LDO52_15340)	22842..23114	+	273	WP_000369781.1	NadS family protein	-
LDO52_RS15345 (LDO52_15345)	23608..25083	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
LDO52_RS15350 (LDO52_15350)	25139..26023	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / floR / blaTEM-2 / aac(3)-IIa / aph(6)-Id / sul2 / dfrA36 / tet(X3) / tet(X6) / lnu(G)	-	1..145524	145524
	-	inside	IScluster/Tn	msr(E) / mph(E) / floR	-	15422..30732	15310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T218417 WP_000286964.1 NZ_CP084302:21306-21608 [Acinetobacter pseudolwoffii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T218417 NZ_CP115941:3099898-3100821 [Ralstonia syzygii]
ATGAATACCACGGCCCCCAAGCATTCTGCGACATCCATCCGGGCGCGGCTCTTCAATCACGCCAAGGAGCGTGGTGACGA
TTTTCAACGCGTTTTGACACGCTACGCAATTGAGCGCCTTCTCTTCCGACTCAGTCAGACAGAAGCCGTCGAGCGCTATG
TGCTCAAGGGCGCCATGCTGTTTGTGACCTGGCCCGAGCACGTCTTTCGCCCATCCGGGGACCTTGATCTACTTGGCCAA
GGCAACCCTGACCCCGACGCGATCACCGAACTCTTCACCCGCATCTGCCAGGTCGACGTCGCGGACGACGGCATCGTTTT
CGAGCCAGCCACGCTGAAGGTCGAACCTGTGCGCGAAGCCGACAAGTACCAAGGGGTTCGATTGAGCCTCAAGGGCGAAC
TGGCCAAGGCGGTTATCCACGTGCAGGTTGACATCGGCTTCGGCGACTACGTCTACCCGCCGCCGAAGTACCACATCTTC
CCAAGCCTACTGCAGGATCTGCCCGCAGCGAACATCCTGATGTACCCACCCGAAACCGTGGTCGCCGAAAAATTCGAGGC
GATGATCCGCTTCGGGGAGGCAAACGGCAGGATCAAGGATTTTCACGACATGTGGGTCATGACCCGCACGTTCTCGTTTG
ATATGCCCACCTTGGTCGAAGCGGTCGGCGGAACACTCCGGCGCCGCGAAACTGCGATCCCGACTGAGATGCCGGCGGGC
CTTACCGAGACGTTCGCGAAGGTCGCCGAGGAGCGCGGCCTTTGGAGCGGCTTCCTGCGCCGAAACCCGCCGACCCTTCA
ACCGCCACCTTTCGCGGACCTGCAAGAGGAGCTACGGCGCTTCTTTGGCCCCGTCATCACCAACCTCGCGCTGCCGGAAG
GCGCCAAAGGCCGATGGGACCCAGATGCCGGAGCTTGGCGCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT218417 WP_000985609.1 NZ_CP084302:21609-21890 [Acinetobacter pseudolwoffii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT218417 NZ_CP115941:3099302-3099901 [Ralstonia syzygii]
ATGACCTATCGCGAGCGCGCCCTCAGTCTCATCCGAGACCGAGGGATCGCGCGCGCGCGCGACTTCAAGGCAGCGGCTAT
CCCCCTGGTCTACCTGAAGCGCCTGACCGATGCCGGCGACCTGGTCCGCCTCGGCCGCGGCCTCTATCAAGACCCGGAGC
GCGTCGGGGAAGACATCGCCCACGATCTGGCGGAAGCCGCCCGCCTCGTCCCCAACGGGGTTATCAGCTTGATCAGCGCC
CTTCGACACCATCAACTGACCACCCAACTGCCGCACGCAGTGTGGATAACCATTCCTCACAAGGCTAGGACACCGAACCT
ACAGGGGCTACGGCTCGAGATCATTCGCGCGACCGACGAAGTCCTGACGGCGGGCGTCGAGCACATCCGCGTAGAGGGGG
TCGACGTGCCGATCTACTGCGTCGCGAAAACAATCGCCGACTGCTTCAAGCATCGCCGCCATGTCGGCGAGGACGTCGCC
ATAGAGGCGCTCCGAGACGCCCTGCGACAAAAGAAGACCACCGTGAGCGAGCTAATGAAATACGCGGCCATTGATCGCGT
CGCCAAACGGATCGAACCCTACATCAAGGCGATGCAATGA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94