218416

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 18463..19051 Replicon plasmid pXMC5X702-tetX-145k
Accession NZ_CP084302
Organism Acinetobacter pseudolwoffii strain XMC5X702

Toxin (Protein)


Gene name brnT Uniprot ID N8SM64
Locus tag LDO52_RS15300 Protein ID WP_000438825.1
Coordinates 18463..18750 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID D3JXQ4
Locus tag LDO52_RS15305 Protein ID WP_004728120.1
Coordinates 18737..19051 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO52_RS15275 (LDO52_15275) 14624..15217 + 594 WP_004758987.1 hypothetical protein -
LDO52_RS16415 15227..15469 - 243 Protein_14 integrase core domain-containing protein -
LDO52_RS15280 (LDO52_15280) 15500..16999 - 1500 Protein_15 IS66-like element ISAba16 family transposase -
LDO52_RS15285 (LDO52_15285) 17143..17478 - 336 WP_000618091.1 IS66 family insertion sequence element accessory protein TnpB -
LDO52_RS15290 (LDO52_15290) 17475..17858 - 384 WP_001055589.1 transposase -
LDO52_RS15295 (LDO52_15295) 17884..18216 + 333 WP_117008139.1 MerR family DNA-binding protein -
LDO52_RS15300 (LDO52_15300) 18463..18750 + 288 WP_000438825.1 BrnT family toxin Toxin
LDO52_RS15305 (LDO52_15305) 18737..19051 + 315 WP_004728120.1 BrnA antitoxin family protein Antitoxin
LDO52_RS15310 (LDO52_15310) 19140..19670 - 531 WP_000172759.1 hypothetical protein -
LDO52_RS15315 (LDO52_15315) 19861..21186 + 1326 WP_001280601.1 ISNCY family transposase -
LDO52_RS15320 (LDO52_15320) 21306..21608 + 303 WP_000286964.1 type II toxin-antitoxin system RelE/ParE family toxin -
LDO52_RS15325 (LDO52_15325) 21609..21890 + 282 WP_000985609.1 putative addiction module antidote protein -
LDO52_RS15330 (LDO52_15330) 21970..22389 - 420 WP_001180106.1 SMI1/KNR4 family protein -
LDO52_RS15335 (LDO52_15335) 22529..22849 + 321 WP_000897307.1 type II toxin-antitoxin system RelE/ParE family toxin -
LDO52_RS15340 (LDO52_15340) 22842..23114 + 273 WP_000369781.1 NadS family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid msr(E) / mph(E) / floR / blaTEM-2 / aac(3)-IIa / aph(6)-Id / sul2 / dfrA36 / tet(X3) / tet(X6) / lnu(G) - 1..145524 145524
- inside IScluster/Tn msr(E) / mph(E) / floR - 15422..30732 15310


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11304.70 Da        Isoelectric Point: 5.6205

>T218416 WP_000438825.1 NZ_CP084302:18463-18750 [Acinetobacter pseudolwoffii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T218416 NZ_CP115941:2607759-2609105 [Ralstonia syzygii]
ATGGGGCGCCGGTCGCATACGCGGGCCCTCGGGCTCTGGATGAATGGCACCCATGTCGGCCACTGGGAGCTGCACCCGCA
TCAAGGCGATGTGCTGAAGTACGCGGCGTCCTGGGTGGCCGCACCCGAGGGGCGGCCCTTGTCGCTATCCCTGCCCTTCA
CGCCGGACAACGCGCCGTACAAAGGCCCGGCGGTCCGGGCATATTTCGAAAACCTCCTGCCGGACAGCCGCGACATCCGC
GAGCGCATCGCGCGCCGGTTCCAGGCGGGCACGACCGAGGCATTCGACCTGCTGGAGCAGATCGGCCGCGACTGCATCGG
CGCCCTGCAGGTGCTGCCCGCCGACATGGCCCCAGCCAGCATCGGCGACATCGACGCGGAGCCGCTGACCGAGGCGCAGA
TCGCGCAGACGCTGCGGGAGACGGTCGCCCCGCCGCCCCTGGGCGGCATGCCGGAGGACGACGGCAATTTCCGCATCTCG
ATTGCCGGCGCGCAGGAAAAGACCGCCTTCCTCCAGATCGACGAACGCTGGTACCGCCCCCGGGGCGCGACGCCGACCAG
CCACATCTTCAAGCTGCCGATGGGGCGGGTCGGCAACATGAAGCTGGACCTGAGCGAGTCGGTGGAAAACGAATGGCTGT
GCTCCCTGGTGCTGCGGGCGTATGGGCTGCCCATCGCCCACTGCCAGCCACTGGTGTTCGAAGACCAGAAAGTCCTGGCC
GTGGAACGGTTCGACCGCATGTGGCGGGACCACCGCGGCAGGCAGCTCATCCGCCTGCCGCAGGAAGACATGTGCCAGGC
GACGGCCACGCCCCCGCACCTGAAGTACGAGGCCGACGGCGGCCCCGGCATCGACCGCATCATGGGCCTGCTCGACGGCT
CCGCGCGCCGGGATGCCGACCGCCTGATGCTGTTCCAGAGCCAGGTGCTGTTCTGGATGCTGTGCGCGCCCGACGGCCAT
GCGAAGAACTTCAGCCTCGCGCTTCGCCCCGGCGGCGCCTACGCGCTCACGCCGCTGTACGACGTGATGTCGGCCTACCC
GCTGCTGGGCGAGGGGCCGGGCAAGCTCTCACCGCACCGGATCCGGCTGGCAATGGCCGTCCGCTCGAAGAACCCGCACT
GGAAAATGCAAGACGTCCAGCGGCGCCACTGGCTGGGCCTGGGCCAGCGGCATGGCATCGTCACGCCCGAGGGCGGGCAT
GCGGCCGCCATCGTGGACAGTCTGGTCGCCCGGACCCCCGAGGTGATCCGCACCGTGCGCGCGCTGCTGCCGGAAGACTT
CCCGCAGCCGCTGGCCGACCGCATTCTGCGGGGGCTGCAGAGCGCCGCGGACAGGCTCGCGCAATAG

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12200.86 Da        Isoelectric Point: 8.4628

>AT218416 WP_004728120.1 NZ_CP084302:18737-19051 [Acinetobacter pseudolwoffii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT218416 NZ_CP115941:2607488-2607754 [Ralstonia syzygii]
ATGAACGAGTTCATCGTACGCACCCCCGAGCAGTTGCCGGCGATTCTCAAGGGCTTCCGCAAACAGGCCGGGCTGAGCCA
GGCTGAACTGGCCACCCGGATGGGCATGCGCCAGCAAACCCTCTCCGCACTGGAGCGCAACGCAGAAAACGTGAGCGCCG
GACGGCTGATGCGCCTGTTGTCGGTGCTGGGCGTCGAGCTGGTGCTGCGCAAACCCGACGCCTCCGGCGAGCGCGGCCGC
CCGGCATCCGACCAGCCGCAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A8TVQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0A8TRI9

References