Detailed information of TA system TA021841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 51354..51937
• Replicon: plasmid pPNAP05
• Accession: NC_008761
• Organism: Polaromonas naphthalenivorans CJ2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A1VX33
• Locus tag: PNAP_RS24560
• Protein ID: WP_011798577.1
• Coordinates: 51354..51734 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A1VX34
• Locus tag: PNAP_RS24565
• Protein ID: WP_011798578.1
• Coordinates: 51731..51937 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PNAP_RS24555	49675..51102	+	1428	Protein_42	IS1182 family transposase	-
PNAP_RS24560	51354..51734	-	381	WP_011798577.1	PIN domain-containing protein	Toxin
PNAP_RS24565	51731..51937	-	207	WP_011798578.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
PNAP_RS25375	52074..53060	-	987	WP_011798579.1	hypothetical protein	-
PNAP_RS24585	53528..54124	-	597	WP_157040568.1	Uma2 family endonuclease	-
PNAP_RS24590	54174..55154	-	981	WP_011798581.1	GIY-YIG nuclease family protein	-
PNAP_RS24595	55422..55634	+	213	WP_041377830.1	helix-turn-helix transcriptional regulator	-
PNAP_RS26595	56019..56228	+	210	WP_083758115.1	DUF4926 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..58808	58808

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13845.01 Da
• Isoelectric Point: 6.2201

>T21841 WP_011798577.1 NC_008761:c51734-51354 [Polaromonas naphthalenivorans CJ2]
MNVLVDTSVWVGHFKQRNERLAALLEDGRVVCHPYVVTEVACGTPPNRRAIIAMLAELESGPLATPDEILELIERRGLYG
RGCGFVDMSLLASALLSDQTLIWTLDKRLESVAAGLNRAYRAALHS

Nucleotide

• Length: 381 bp

>T21841 NC_008761:c51734-51354 [Polaromonas naphthalenivorans CJ2]
ATGAACGTACTGGTCGATACCTCTGTGTGGGTGGGCCACTTCAAGCAGCGCAATGAGCGCTTGGCCGCTTTGCTCGAAGA
CGGCAGGGTCGTATGCCACCCCTATGTGGTCACGGAAGTGGCCTGCGGGACACCTCCGAACCGGCGCGCCATCATTGCCA
TGCTGGCCGAACTTGAAAGCGGGCCGCTTGCGACACCGGATGAGATTTTGGAATTGATCGAGCGTCGGGGCCTCTACGGC
CGGGGGTGTGGCTTCGTTGACATGAGCCTGCTGGCTTCGGCGCTATTGAGCGATCAGACCTTGATCTGGACTTTGGACAA
GCGTCTGGAGTCCGTGGCGGCCGGGCTGAACCGGGCCTACCGTGCGGCACTTCACTCCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7473.60 Da
• Isoelectric Point: 7.9972

>AT21841 WP_011798578.1 NC_008761:c51937-51731 [Polaromonas naphthalenivorans CJ2]
MRTTLTIDDQLFARAVALAEPGIEKSELIRECVKAFIQRQAARRLAALGGQAPDIELAPRRREDAPAS

Nucleotide

• Length: 207 bp

>AT21841 NC_008761:c51937-51731 [Polaromonas naphthalenivorans CJ2]
ATGCGAACCACACTCACGATTGATGATCAACTTTTTGCCAGGGCCGTGGCACTGGCCGAGCCGGGGATCGAGAAGTCCGA
GCTGATCAGGGAATGCGTCAAGGCGTTCATTCAGCGCCAGGCGGCCCGTCGCTTGGCGGCCCTGGGCGGGCAGGCGCCTG
ATATTGAACTCGCGCCTCGCCGGCGCGAAGACGCGCCCGCTTCATGA

Structures

Toxin

• AlphaFold DB: A1VX33

Antitoxin

• AlphaFold DB: A1VX34