Detailed information of TA system TA218406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2326008..2326652
• Replicon: chromosome
• Accession: NZ_CP084296
• Organism: Providencia alcalifaciens strain LHC2-1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: B6XA97
• Locus tag: LDO51_RS10620
• Protein ID: WP_004905417.1
• Coordinates: 2326008..2326211 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LDO51_RS10625
• Protein ID: WP_225574546.1
• Coordinates: 2326284..2326652 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO51_RS10600 (LDO51_10600)	2321862..2323646	+	1785	WP_225574543.1	SmdB family multidrug efflux ABC transporter permease/ATP-binding protein	-
LDO51_RS10605 (LDO51_10605)	2323798..2324664	-	867	WP_225574544.1	acyl-CoA thioesterase II	-
LDO51_RS10610 (LDO51_10610)	2324903..2325361	+	459	WP_225574545.1	YbaY family lipoprotein	-
LDO51_RS10620 (LDO51_10620)	2326008..2326211	-	204	WP_004905417.1	HHA domain-containing protein	Toxin
LDO51_RS10625 (LDO51_10625)	2326284..2326652	-	369	WP_225574546.1	Hha toxicity modulator TomB	Antitoxin
LDO51_RS10630 (LDO51_10630)	2327168..2328553	-	1386	WP_225574547.1	murein transglycosylase D	-
LDO51_RS10635 (LDO51_10635)	2328636..2329388	-	753	WP_225574548.1	hydroxyacylglutathione hydrolase	-
LDO51_RS10640 (LDO51_10640)	2329423..2330148	+	726	WP_225574549.1	methyltransferase domain-containing protein	-
LDO51_RS10645 (LDO51_10645)	2330161..2330631	-	471	WP_036953952.1	ribonuclease HI	-
LDO51_RS10650 (LDO51_10650)	2330686..2331447	+	762	WP_225574550.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8061.34 Da
• Isoelectric Point: 6.9770

>T218406 WP_004905417.1 NZ_CP084296:c2326211-2326008 [Providencia alcalifaciens]
MTKSDYLMRLRKCTTIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Nucleotide

• Length: 204 bp

>T218406 NZ_CP115930:c4665489-4664956 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 4.4687

>AT218406 WP_225574546.1 NZ_CP084296:c2326652-2326284 [Providencia alcalifaciens]
MDEYSPKKHDIAELKYLCNSLNRDAISSLLKTNTHWVNDLSSAQSISLNELVEHIAAFVWRFKIKYPKENLVISLVEEYL
DETYNLFGSPVITFSEIIDWESMNQNLVAVLDDDLKCLTSKT

Nucleotide

• Length: 369 bp

>AT218406 NZ_CP115930:c4665766-4665500 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A291E6Q2