Detailed information of TA system TA218402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 972745..973394
• Replicon: chromosome
• Accession: NZ_CP084296
• Organism: Providencia alcalifaciens strain LHC2-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LDO51_RS04360
• Protein ID: WP_036949118.1
• Coordinates: 972745..973164 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6L6FAJ1
• Locus tag: LDO51_RS04365
• Protein ID: WP_036949116.1
• Coordinates: 973161..973394 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO51_RS04335 (LDO51_04335)	968020..968727	+	708	WP_225577218.1	phosphonate utilization transcriptional regulator PhnR	-
LDO51_RS04340 (LDO51_04340)	968864..969877	+	1014	WP_225577219.1	2-aminoethylphosphonate ABC transporter substrate-binding protein	-
LDO51_RS04345 (LDO51_04345)	969885..970994	+	1110	WP_225576493.1	2-aminoethylphosphonate ABC transport system ATP-binding subunit PhnT	-
LDO51_RS04350 (LDO51_04350)	970996..971856	+	861	WP_225576494.1	2-aminoethylphosphonate ABC transporter permease subunit	-
LDO51_RS04355 (LDO51_04355)	971859..972656	+	798	WP_225576495.1	2-aminoethylphosphonate ABC transport system, membrane component PhnV	-
LDO51_RS04360 (LDO51_04360)	972745..973164	-	420	WP_036949118.1	type II toxin-antitoxin system VapC family toxin	Toxin
LDO51_RS04365 (LDO51_04365)	973161..973394	-	234	WP_036949116.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LDO51_RS04375 (LDO51_04375)	973723..974658	+	936	WP_154603006.1	aspartate carbamoyltransferase	-
LDO51_RS04380 (LDO51_04380)	974669..975133	+	465	WP_036949112.1	aspartate carbamoyltransferase regulatory subunit	-
LDO51_RS04385 (LDO51_04385)	975226..975612	+	387	WP_225576497.1	2-iminobutanoate/2-iminopropanoate deaminase	-
LDO51_RS04390 (LDO51_04390)	975943..978081	+	2139	WP_225576498.1	anaerobic ribonucleoside-triphosphate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-128)

Antitoxin

• SpoVT-AbrB domain (9-54)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15496.96 Da
• Isoelectric Point: 7.2913

>T218402 WP_036949118.1 NZ_CP084296:c973164-972745 [Providencia alcalifaciens]
VTKLYMLDTNICSFIMREQPMYLLQKLQDCVMHRHSIVISAITYSEMRFGAIGKKASPKHNFLVDAFCERLDGILAWDKD
AIDATTAIKKALTEAGTPIGNNDTAIAGHALATNSILVTNNTREFSRVLGLKLEDWLQP

Nucleotide

• Length: 420 bp

>T218402 NZ_CP115930:872098-872508 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8932.26 Da
• Isoelectric Point: 4.8660

>AT218402 WP_036949116.1 NZ_CP084296:c973394-973161 [Providencia alcalifaciens]
MVTKIVAVFKNGNNRAVRLPKDIDFEGVNELEISREGNRIILRPVKPNWLSLRDEDKADKDFLLEREDVIEDGRIVL

Nucleotide

• Length: 234 bp

>AT218402 NZ_CP115930:871851-872117 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6L6FAJ1