Detailed information of TA system TA218400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4750638..4751254
• Replicon: chromosome
• Accession: NZ_CP084290
• Organism: Citrobacter werkmanii strain LHC5-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LD024_RS22550
• Protein ID: WP_227410076.1
• Coordinates: 4750638..4751012 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8E9YHE9
• Locus tag: LD024_RS22555
• Protein ID: WP_001523745.1
• Coordinates: 4751012..4751254 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LD024_RS22535 (4748141)	4748141..4749043	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
LD024_RS22540 (4749040)	4749040..4749675	+	636	WP_038635897.1	formate dehydrogenase cytochrome b556 subunit	-
LD024_RS22545 (4749672)	4749672..4750601	+	930	WP_006686145.1	formate dehydrogenase accessory protein FdhE	-
LD024_RS22550 (4750638)	4750638..4751012	-	375	WP_227410076.1	type II toxin-antitoxin system VapC family toxin	Toxin
LD024_RS22555 (4751012)	4751012..4751254	-	243	WP_001523745.1	CopG family transcriptional regulator	Antitoxin
LD024_RS22560 (4751458)	4751458..4752366	+	909	WP_227410077.1	alpha/beta hydrolase	-
LD024_RS22565 (4752431)	4752431..4752673	+	243	WP_069324888.1	type II toxin-antitoxin system ParD family antitoxin	-
LD024_RS22570 (4752666)	4752666..4752953	+	288	WP_085049709.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LD024_RS22575 (4752959)	4752959..4753900	-	942	WP_032940213.1	fatty acid biosynthesis protein FabY	-
LD024_RS22580 (4753945)	4753945..4754382	-	438	WP_003825289.1	D-aminoacyl-tRNA deacylase	-
LD024_RS22585 (4754379)	4754379..4755251	-	873	WP_085049708.1	virulence factor BrkB family protein	-
LD024_RS22590 (4755245)	4755245..4755844	-	600	WP_069324890.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13635.85 Da
• Isoelectric Point: 8.5950

>T218400 WP_227410076.1 NZ_CP084290:c4751012-4750638 [Citrobacter werkmanii]
MVKGAALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRIALGAFNIIGVSQDIAERSVSI
RQEQGMKLPDAIILATAQIHHLTLVTRNTKDFASISGVVTPYTL

Nucleotide

• Length: 375 bp

>T218400 NZ_CP115930:351707-352054 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.77 Da
• Isoelectric Point: 4.4463

>AT218400 WP_001523745.1 NZ_CP084290:c4751254-4751012 [Citrobacter werkmanii]
MMAGMDMGRILLDLSDDVIKRLDDLKVQRNLPRAELLREAVEQYLERQDRAETTISKALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT218400 NZ_CP115930:352054-352353 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA