Detailed information of TA system TA218398

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 4463259..4464052
• Replicon: chromosome
• Accession: NZ_CP084290
• Organism: Citrobacter werkmanii strain LHC5-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: LD024_RS21215
• Protein ID: WP_000854726.1
• Coordinates: 4463675..4464052 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: LD024_RS21210
• Protein ID: WP_175330022.1
• Coordinates: 4463259..4463585 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LD024_RS21165 (4458695)	4458695..4459516	+	822	WP_227410038.1	DUF932 domain-containing protein	-
LD024_RS21170 (4459734)	4459734..4460435	+	702	WP_175330025.1	WYL domain-containing protein	-
LD024_RS21175 (4460476)	4460476..4460712	+	237	WP_001144031.1	protein YpjK	-
LD024_RS21180 (4460712)	4460712..4461155	+	444	WP_175330024.1	hypothetical protein	-
LD024_RS21185 (4461179)	4461179..4461646	+	468	WP_001547765.1	protein YkfB	-
LD024_RS21190 (4461723)	4461723..4461962	+	240	WP_000194654.1	DUF905 family protein	-
LD024_RS21195 (4462060)	4462060..4462518	+	459	WP_000211838.1	antirestriction protein	-
LD024_RS21200 (4462534)	4462534..4463010	+	477	WP_175330023.1	RadC family protein	-
LD024_RS21205 (4463019)	4463019..4463240	+	222	WP_000691994.1	DUF987 domain-containing protein	-
LD024_RS21210 (4463259)	4463259..4463585	+	327	WP_175330022.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
LD024_RS21215 (4463675)	4463675..4464052	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
LD024_RS21220 (4464049)	4464049..4464537	+	489	WP_227410039.1	DUF5983 family protein	-
LD024_RS21225 (4464549)	4464549..4464746	+	198	WP_115799768.1	DUF957 domain-containing protein	-
LD024_RS21230 (4464831)	4464831..4465673	+	843	WP_001548928.1	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T218398 WP_000854726.1 NZ_CP084290:4463675-4464052 [Citrobacter werkmanii]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T218398 NZ_CP115926:297113-297610 [Agrobacterium fabacearum]
GTGACTCTCAGCGCACCAGTCCCCCTGGCCGATCATCACGAACTGGCCGAATTCAATTCTGGAGTGCCTGAACTGGACGA
CTGGCTTCGGCGCCGTGCCCGGGCCAATCAAGCAGGCGGCGCGTCGCGGACCTTTGTCGTGTGCGACGAGAGCCGCGTCA
TCGCGTACTACGCCCTGGCCTCTGGCTCCGTGAAACCGCCGGAAGTGCCTGGTCGCTTTCGGCGCAATATGCCTGATCCA
ATCCCAGTTGCAGTGCTTGGCCGCCTCGCCATTGATCAATCCTGTCAAGGGCGTGGTATCGGCAGGGCATTGGTGCGCGA
CGCTGGTTTGCGCCTGCTGAATGCCGCTGAAGTGCTCGGGATCCGCGGCGTGCTGGTCCATGCGATTTCTGATGACGCAC
GAGCCTTCTACGAAGCCGTCGGCTTCCTGTCTTCTCCGTCCGATCCCATGATGCTGATGGTCGGTTTGCACGACCTCAAT
AACGCGCTGAATACCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12278.00 Da
• Isoelectric Point: 7.7489

>AT218398 WP_175330022.1 NZ_CP084290:4463259-4463585 [Citrobacter werkmanii]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDKTFPHFIRQMESMLTTGELSPNHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT218398 NZ_CP115926:296820-297116 [Agrobacterium fabacearum]
ATGCCGACCTCACACACGGGTAAGAGCCCGGACGCAAGTGTGCCGCTGAATATGCGCATCAAACCTGCTACTCGCAACTT
GATAGATCGCGCCGCAGAGTTGCTCGGCAAAACGCGCACAGATTTCATGTTGGAAGCTTCCGAGCGCCGTGCGGAGGAGG
TGCTGCTCGACCGTACAATCGTCACTGTAAGCCCCGAAATTTATGCTGAATATCTTGCCCGTCTCGATGCGCCTGCACAG
CCCAATGAGCGTCTAAAGCGCACGATGTCGACCAAAGCGCCCTGGGATGAGGCGTGA

Structures

Toxin

• AlphaFold DB: S1EZ92