Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3642041..3642661 Replicon chromosome
Accession NZ_CP084290
Organism Citrobacter werkmanii strain LHC5-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LD024_RS17310 Protein ID WP_002892050.1
Coordinates 3642443..3642661 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag LD024_RS17305 Protein ID WP_003021733.1
Coordinates 3642041..3642415 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD024_RS17295 (3637188) 3637188..3638381 + 1194 WP_204526591.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LD024_RS17300 (3638404) 3638404..3641553 + 3150 WP_038638345.1 efflux RND transporter permease AcrB -
LD024_RS17305 (3642041) 3642041..3642415 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
LD024_RS17310 (3642443) 3642443..3642661 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LD024_RS17315 (3642847) 3642847..3643398 + 552 WP_121528689.1 maltose O-acetyltransferase -
LD024_RS17320 (3643515) 3643515..3643985 + 471 WP_069323967.1 YlaC family protein -
LD024_RS17325 (3644059) 3644059..3644199 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
LD024_RS17330 (3644201) 3644201..3644461 - 261 WP_038638331.1 type B 50S ribosomal protein L31 -
LD024_RS17335 (3644650) 3644650..3646203 + 1554 WP_227409911.1 EAL domain-containing protein -
LD024_RS17340 (3646255) 3646255..3646608 - 354 WP_042310059.1 DUF1428 family protein -
LD024_RS17345 (3646673) 3646673..3647302 - 630 WP_087857057.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T218396 WP_002892050.1 NZ_CP084290:3642443-3642661 [Citrobacter werkmanii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T218396 NZ_CP115926:219047-219328 [Agrobacterium fabacearum]
ATGATCCGTTCTTTCAAAAACAGGCTCACGTCGTCAATCGATGACGGATCAGTGAAGAAGGGATTTCCCGCCGATCTGGT
ACGTCGCGCCCAGCAATTGCTAACCATTCTGGACGCGGCAACCACGGTTGAAGACCTTCGCTCCCCGCCCGGTAACCGCC
TGGAAAAGCTATTCGGTGATCGCGAAGGTCAACATTCGATCAGGATCAACAAACAATGGCGCATCTGCTTTGTCTGGACA
GAGGCAGGGCCGGACAACGTAGAAATCACCGACTATCATTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT218396 WP_003021733.1 NZ_CP084290:3642041-3642415 [Citrobacter werkmanii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT218396 NZ_CP115926:219384-219683 [Agrobacterium fabacearum]
ATGACAATCACCAAATCTCCCGCGATCCATCCGGGCGAAATTCTGCGGGAACTTTACCTTGAGCCGCTGGACATGTCAGC
CTACGCGCTTGCGAAGCACCTCAATATTCCCCGCACCCGCATTGAGCGGATCGTGGCGGAGAAGCTTGGCATCACGGCTG
ACACCGCCTTGCGGCTGGCGAAGTTCTTCAAGACCACGCCGGAGTACTGGCTGAACTTGCAGAACGCTTTTGATATCAAG
AGCGCCGCCGCTATGCTGGAGGCCGATCTTGCCGCTATCAAGGAACTGGAAGCGGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References