Detailed information of TA system TA218385

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 803853..804646
• Replicon: chromosome
• Accession: NZ_CP084290
• Organism: Citrobacter werkmanii strain LHC5-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: LD024_RS03915
• Protein ID: WP_000854726.1
• Coordinates: 803853..804230 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: LD024_RS03920
• Protein ID: WP_175330022.1
• Coordinates: 804320..804646 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LD024_RS03885 (799405)	799405..799938	+	534	WP_121529532.1	type VI secretion system lipoprotein TssJ	-
LD024_RS03890 (799938)	799938..800381	+	444	WP_227410238.1	type VI secretion system baseplate subunit TssE	-
LD024_RS03895 (800383)	800383..801723	+	1341	WP_199952306.1	VasL domain-containing protein	-
LD024_RS03900 (802232)	802232..803074	-	843	WP_001548928.1	DUF4942 domain-containing protein	-
LD024_RS03905 (803159)	803159..803356	-	198	WP_115799768.1	DUF957 domain-containing protein	-
LD024_RS03910 (803368)	803368..803856	-	489	WP_227410039.1	DUF5983 family protein	-
LD024_RS03915 (803853)	803853..804230	-	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
LD024_RS03920 (804320)	804320..804646	-	327	WP_175330022.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
LD024_RS03925 (804665)	804665..804886	-	222	WP_000691994.1	DUF987 domain-containing protein	-
LD024_RS03930 (804895)	804895..805371	-	477	WP_175330023.1	RadC family protein	-
LD024_RS03935 (805387)	805387..805845	-	459	WP_000211838.1	antirestriction protein	-
LD024_RS03940 (805943)	805943..806182	-	240	WP_000194654.1	DUF905 family protein	-
LD024_RS03945 (806259)	806259..806726	-	468	WP_001547765.1	protein YkfB	-
LD024_RS03950 (806750)	806750..807193	-	444	WP_175330024.1	hypothetical protein	-
LD024_RS03955 (807193)	807193..807429	-	237	WP_001144031.1	protein YpjK	-
LD024_RS03960 (807470)	807470..808171	-	702	WP_175330025.1	WYL domain-containing protein	-
LD024_RS03965 (808389)	808389..809210	-	822	WP_227410038.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T218385 WP_000854726.1 NZ_CP084290:c804230-803853 [Citrobacter werkmanii]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T218385 NZ_CP115911:427255-427407 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12278.00 Da
• Isoelectric Point: 7.7489

>AT218385 WP_175330022.1 NZ_CP084290:c804646-804320 [Citrobacter werkmanii]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDKTFPHFIRQMESMLTTGELSPNHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT218385 NZ_CP115911:427471-427605 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Structures

Toxin

• AlphaFold DB: S1EZ92