Detailed information of TA system TA218384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 282300..282931
• Replicon: chromosome
• Accession: NZ_CP084290
• Organism: Citrobacter werkmanii strain LHC5-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0J1MPQ6
• Locus tag: LD024_RS01340
• Protein ID: WP_003837806.1
• Coordinates: 282300..282575 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LD024_RS01345
• Protein ID: WP_087856264.1
• Coordinates: 282572..282931 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LD024_RS01330 (278351)	278351..281098	+	2748	WP_227410160.1	ribosome-associated ATPase/putative transporter RbbA	-
LD024_RS01335 (281098)	281098..282222	+	1125	WP_038635040.1	ABC transporter permease	-
LD024_RS01340 (282300)	282300..282575	+	276	WP_003837806.1	type II toxin-antitoxin system HicA family toxin	Toxin
LD024_RS01345 (282572)	282572..282931	+	360	WP_087856264.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LD024_RS01350 (283045)	283045..283446	-	402	WP_006687486.1	nickel-responsive transcriptional regulator NikR	-
LD024_RS01355 (283461)	283461..283541	-	81	Protein_266	nickel import ATP-binding protein NikE	-
LD024_RS01360 (283538)	283538..284398	-	861	WP_038635037.1	LysR family transcriptional regulator	-
LD024_RS01365 (284498)	284498..285004	+	507	WP_227410161.1	phenolic acid decarboxylase	-
LD024_RS01370 (284996)	284996..285586	-	591	WP_038635032.1	4'-phosphopantetheinyl transferase AcpT	-
LD024_RS01375 (285649)	285649..286698	-	1050	WP_032940622.1	AI-2E family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10210.88 Da
• Isoelectric Point: 10.7228

>T218384 WP_003837806.1 NZ_CP084290:282300-282575 [Citrobacter werkmanii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T218384 NZ_CP115905:1996-2274 [Neisseria gonorrhoeae]
ATGTACGCGATTTCTTTTGATTTGGTGGTTGCCGATACCGCTCAAAACCATCCGAAAGGCATTTCCCAAGCCTACGCGGA
TATTGGATACACCCTGCGGAAATTCGGCTTTACCCGCATTCAGGGAAGTTTGTACACCTGCCAAAACGAAGATATGGCTA
ACCTGTTTTCAGCCATCAACGAGCTAAAAGCTCTGCCTTGGTTTCCGTCTTCTGTCCGCGATATTCGGGCGTTCCGCATT
GAGCAGTGGTCTGATTTCACAAGTCTTGTGAAGTCTTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13206.02 Da
• Isoelectric Point: 4.7022

>AT218384 WP_087856264.1 NZ_CP084290:282572-282931 [Citrobacter werkmanii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVADSIQGLQKEGEISLREYLDDCSAAGIEPYAHPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT218384 NZ_CP115905:1811-1996 [Neisseria gonorrhoeae]
ATGCAAACCACTATCCCCACCGGTTCAATCCGCACCTTTGGCGATTACGGCGTGATGTACATCGTCGGCACGCCTGCCGA
ACAGCTTCCGGACGGCGATTGGCTCGTAAATATTGAACTGCCGGAAAGCGGCGAACACACGCAATACAAACTGTCCCACA
TCGTTCAAGACCCAAAGGCTGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0J1MPQ6