Detailed information of TA system TA218383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 71300..72972
• Replicon: plasmid p2
• Accession: NZ_CP084288
• Organism: Caballeronia zhejiangensis strain A33_M4_a

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LDZ23_RS33155
• Protein ID: WP_033535671.1
• Coordinates: 71641..72972 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A656QT45
• Locus tag: LDZ23_RS33150
• Protein ID: WP_008345344.1
• Coordinates: 71300..71641 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDZ23_RS33125	66669..67388	-	720	WP_008345349.1	response regulator transcription factor	-
LDZ23_RS33130	67776..68921	+	1146	WP_008345348.1	porin	-
LDZ23_RS33135	68985..69383	-	399	Protein_66	fumarylacetoacetate hydrolase family protein	-
LDZ23_RS33140	69326..69877	-	552	WP_008345346.1	ThiF family adenylyltransferase	-
LDZ23_RS33145	69874..71172	-	1299	WP_051078946.1	CoA-transferase	-
LDZ23_RS33150	71300..71641	+	342	WP_008345344.1	helix-turn-helix transcriptional regulator	Antitoxin
LDZ23_RS33155	71641..72972	+	1332	WP_033535671.1	type II toxin-antitoxin system HipA family toxin	Toxin
LDZ23_RS33160	72982..74289	-	1308	WP_008345342.1	chloride channel protein	-
LDZ23_RS33165	74338..74736	-	399	WP_008345341.1	MarR family winged helix-turn-helix transcriptional regulator	-
LDZ23_RS33170	74786..75187	-	402	WP_008345340.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..439699	439699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (11-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-67)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49540.83 Da
• Isoelectric Point: 9.6735

>T218383 WP_033535671.1 NZ_CP084288:71641-72972 [Caballeronia zhejiangensis]
IMARRTQSGRLDLWMNGLPVGHWETGAAGERLVYRDDWIADPQGRPLSLSLPFTPGNQPWRGQVVTDFFDNLLPDSEPIR
RRIATRFRTGGTAPFQLLAQLGRDCVGALQMLPPGEAPVDLDTIKGRALTASQIARLLRETTSAPQLGQHETVDDLRLSI
AGAQEKTALLRHGKRWLLPEGSTPTTHIFKLPLGLVGNMRADMRTSVENEWLCSKIVAAFGLPIARCEMERFDDQKVLVV
ERFDRRLSSDKSWILRLPQEDMCQATGTPALLKYESDGGPGIERIMEVLAGSTHAAQDRLRFFRTQMIFWILAATDGHAK
NFSIAHLPGNRYESTPLYDVLSAHPVIGTRANQFAAQRVRLAMAVRSKNAHYLIGEIQRRHWIAQGRRVGLAANDVETKI
DELGARTPHVIDEVAARLPRDFPMDVAETIFDGMRRLARKLAS

Nucleotide

• Length: 1332 bp

>T218383 NZ_CP115904:c1665438-1665256 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12099.11 Da
• Isoelectric Point: 11.3927

>AT218383 WP_008345344.1 NZ_CP084288:71300-71641 [Caballeronia zhejiangensis]
MDYSLKTLSQLRPILIGFRKAAGLTQAAVAARLGVTQQTYAQLEANPAAVSVERLFKVLRVLDVELALTRAEKASEATPI
HPPPPIAAKATAARPARKRATAAAAASKKRENW

Nucleotide

• Length: 342 bp

>AT218383 NZ_CP115904:c1665154-1664753 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Antitoxin

• AlphaFold DB: A0A656QT45