Detailed information of TA system TA021838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 138452..139958
• Replicon: plasmid pPNAP02
• Accession: NC_008758
• Organism: Polaromonas naphthalenivorans CJ2

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A1VW15
• Locus tag: PNAP_RS22710
• Protein ID: WP_011798122.1
• Coordinates: 138452..139333 (-)
• Length: 294 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: PNAP_RS22715
• Protein ID: WP_041377697.1
• Coordinates: 139353..139958 (-)
• Length: 202 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PNAP_RS22695	136180..136557	+	378	WP_011798125.1	hypothetical protein	-
PNAP_RS26310	136640..137125	-	486	WP_011798124.1	hypothetical protein	-
PNAP_RS27335	137091..137228	-	138	WP_157040501.1	hypothetical protein	-
PNAP_RS22705	137238..138251	-	1014	WP_011798123.1	integrase	-
PNAP_RS22710	138452..139333	-	882	WP_011798122.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
PNAP_RS22715	139353..139958	-	606	WP_041377697.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
PNAP_RS22720	140215..141479	+	1265	Protein_140	lytic murein transglycosylase	-
PNAP_RS22725	141836..142213	-	378	WP_011798120.1	hypothetical protein	-
PNAP_RS22730	142679..143044	+	366	WP_011798119.1	H-NS histone family protein	-
PNAP_RS22740	143907..144353	+	447	WP_011798118.1	RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..190172	190172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-257)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (8-55)

Sequences

Toxin

Protein

• Length: 294 a.a.
• Molecular weight: 32153.29 Da
• Isoelectric Point: 7.0642

>T21838 WP_011798122.1 NC_008758:c139333-138452 [Polaromonas naphthalenivorans CJ2]
MTHDIAASVRARLLNLAKAEGSEFNQVLVRYTLERLLYRLSQSAHADRFVLKGALLFTLWYDMAHRPTRDADLLGFGPSD
LASIDQMFRDIAGVAVEDGIVFEPASVKVEDIRKESGYGGARVLITGELAKARCRAQVDIGFGDAVTPGPVQAVFPVLLA
DFPAPQLRTYPVYTVIAEKLHAIALLGMTNTRLKDYLDLSVLLEREALDPTILATAIAATFMRRGMAVPAQLPIGLSDEF
ANDATRQALWRAFLKKNALTITPLPDTVSALHAYLHPVIRQAALQQMDLSKTL

Nucleotide

• Length: 882 bp

>T21838 NC_008758:c139333-138452 [Polaromonas naphthalenivorans CJ2]
ATGACCCATGACATTGCCGCTTCCGTGCGCGCACGCCTGCTCAACCTGGCCAAGGCCGAAGGTTCTGAGTTCAACCAGGT
GCTGGTTCGCTACACGCTCGAGCGCCTGCTGTACCGCCTGAGCCAGTCCGCGCACGCCGACCGCTTTGTGCTCAAGGGCG
CGTTGCTGTTCACGCTGTGGTACGACATGGCGCACCGCCCGACCCGGGACGCCGACCTGCTGGGCTTTGGCCCAAGCGAC
CTGGCCTCTATCGACCAGATGTTTCGCGACATCGCTGGCGTTGCCGTCGAGGATGGCATCGTCTTTGAGCCGGCCAGCGT
CAAGGTCGAGGACATCCGCAAGGAATCCGGTTACGGCGGCGCGCGGGTGCTGATCACGGGCGAGCTGGCAAAGGCACGCT
GCCGGGCCCAAGTGGACATCGGCTTTGGCGACGCGGTCACGCCAGGGCCGGTGCAGGCGGTGTTTCCCGTGTTGCTGGCA
GATTTTCCCGCACCACAGCTCAGAACCTACCCGGTGTACACAGTCATTGCCGAAAAGCTCCACGCTATCGCGCTGCTGGG
CATGACCAACACCCGGTTGAAGGACTACCTGGATTTGTCGGTCCTGCTGGAGCGAGAGGCGCTGGACCCGACGATCTTGG
CGACAGCGATCGCTGCCACGTTCATGCGCCGGGGCATGGCGGTGCCGGCGCAACTGCCCATCGGGCTGAGCGATGAGTTT
GCCAACGATGCGACGCGCCAGGCGCTGTGGCGTGCCTTTCTCAAGAAGAACGCGCTGACCATCACGCCGCTGCCGGACAC
GGTATCGGCTCTGCACGCTTACCTTCATCCGGTGATCAGACAAGCTGCGCTGCAGCAAATGGATTTGTCGAAGACACTTT
AA

Antitoxin

Protein

• Length: 202 a.a.
• Molecular weight: 22708.51 Da
• Isoelectric Point: 10.6413

>AT21838 WP_041377697.1 NC_008758:c139958-139353 [Polaromonas naphthalenivorans CJ2]
MPQPTQTQRVLELARQHGLLRSRDLQAVGAPRVMLTRLMASGQLEKVGRGLYRLPQTQMSEDESLAAIAIKVPQAVFCLL
TALQFHGLTTQLPRQVWLAMPRGSHAPRIDYPPIRMVQYSGQSYSQGIEVFERDRVLLRVYSVAKTVVDCFKHRSAIGLD
VALEALREARASQRARADELWRFAKICRVANVMRPYLEAIG

Nucleotide

• Length: 606 bp

>AT21838 NC_008758:c139958-139353 [Polaromonas naphthalenivorans CJ2]
ATGCCGCAGCCCACGCAGACCCAGCGCGTTCTAGAACTGGCCCGCCAGCACGGCCTGCTGCGCTCGCGTGACCTTCAAGC
GGTGGGCGCGCCCCGGGTGATGCTGACCCGCTTGATGGCCAGCGGCCAGCTGGAAAAAGTGGGCCGCGGCCTGTACCGCC
TGCCCCAAACACAGATGTCGGAGGACGAAAGCCTGGCTGCCATTGCGATCAAGGTGCCGCAGGCCGTATTTTGCCTGCTC
ACGGCGCTGCAGTTTCACGGCCTGACCACGCAGCTGCCGCGCCAGGTCTGGCTGGCCATGCCGCGTGGCAGCCATGCGCC
GCGCATCGACTATCCGCCCATCCGGATGGTGCAGTATTCGGGTCAATCGTATTCCCAAGGCATCGAGGTGTTCGAGCGCG
ACCGGGTGCTGCTGCGGGTCTACAGCGTGGCCAAGACGGTGGTCGATTGCTTCAAGCACCGCAGCGCCATTGGCCTGGAC
GTGGCGCTCGAAGCGCTCAGAGAAGCAAGAGCCAGCCAACGGGCCCGCGCCGACGAGCTCTGGCGCTTTGCCAAGATTTG
CCGCGTTGCCAATGTGATGCGTCCCTACCTGGAGGCGATTGGCTGA

Structures

Toxin

• AlphaFold DB: A1VW15