Detailed information of TA system TA218369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/-
• Location: 2081880..2082577
• Replicon: chromosome
• Accession: NZ_CP084248
• Organism: Enterococcus lactis strain DH9003

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: K9N64_RS12560
• Protein ID: WP_225479451.1
• Coordinates: 2082158..2082577 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: K9N64_RS12555
• Protein ID: WP_002316334.1
• Coordinates: 2081880..2082134 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9N64_RS12525 (K9N64_12505)	2077326..2078492	-	1167	WP_002292137.1	radical SAM family heme chaperone HemW	-
K9N64_RS12530 (K9N64_12510)	2078508..2078735	-	228	Protein_1947	hypothetical protein	-
K9N64_RS12535 (K9N64_12515)	2078987..2079253	-	267	WP_002292138.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K9N64_RS12540 (K9N64_12520)	2079250..2079480	-	231	WP_002292139.1	DUF6290 family protein	-
K9N64_RS12545 (K9N64_12525)	2079972..2080637	-	666	WP_002292140.1	hypothetical protein	-
K9N64_RS12550 (K9N64_12530)	2080774..2081733	-	960	WP_000222573.1	IS30 family transposase	-
K9N64_RS12555 (K9N64_12535)	2081880..2082134	+	255	WP_002316334.1	hypothetical protein	Antitoxin
K9N64_RS12560 (K9N64_12540)	2082158..2082577	+	420	WP_225479451.1	toxin	Toxin
K9N64_RS12565 (K9N64_12545)	2082809..2083567	-	759	WP_167779246.1	restriction endonuclease	-
K9N64_RS12570	2083759..2084013	+	255	WP_087063755.1	toxin	-
K9N64_RS12575 (K9N64_12550)	2084066..2084497	-	432	WP_002342168.1	N-6 DNA methylase	-
K9N64_RS12580 (K9N64_12555)	2084707..2084868	-	162	WP_002342167.1	hypothetical protein	-
K9N64_RS12585 (K9N64_12560)	2084861..2085529	-	669	WP_225479172.1	zeta toxin family protein	-
K9N64_RS12590 (K9N64_12565)	2086254..2086882	+	629	Protein_1959	glutamine amidotransferase	-
K9N64_RS12595 (K9N64_12570)	2087053..2087496	-	444	WP_002345979.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2080774..2081733	959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 16460.51 Da
• Isoelectric Point: 4.6731

>T218369 WP_225479451.1 NZ_CP084248:2082158-2082577 [Enterococcus lactis]
VISEGLYFEAADLANELSEEVSYYCSKKMEHVKCYDIENYVNEVEDVEFIDYDFKDNLSKKMLGSITKVHGEIIITTNQH
LMLERKNFTKMHEIIHYYKDVPYLGESHAFSDMILEDTYLPEDYKKTSSKYRCQYPHGE

Nucleotide

• Length: 420 bp

>T218369 NZ_CP115887:c2050354-2050178 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9799.93 Da
• Isoelectric Point: 4.2290

>AT218369 WP_002316334.1 NZ_CP084248:2081880-2082134 [Enterococcus lactis]
MSNYQLVTVIADFFNVSTDFLLNRTSDPTPPKRSEEDQLGDSILSHFRLNTADMEVEDIEELEEELNEYMDFLVQRAKAK
KNKK

Nucleotide

• Length: 255 bp

>AT218369 NZ_CP115887:2050055-2050110 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG