Detailed information of TA system TA218368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 2078987..2079480
• Replicon: chromosome
• Accession: NZ_CP084248
• Organism: Enterococcus lactis strain DH9003

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K9N64_RS12535
• Protein ID: WP_002292138.1
• Coordinates: 2078987..2079253 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K9N64_RS12540
• Protein ID: WP_002292139.1
• Coordinates: 2079250..2079480 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9N64_RS12515 (K9N64_12495)	2075392..2075955	-	564	WP_002292135.1	nucleotide exchange factor GrpE	-
K9N64_RS12520 (K9N64_12500)	2076049..2077092	-	1044	WP_225479171.1	heat-inducible transcriptional repressor HrcA	-
K9N64_RS12525 (K9N64_12505)	2077326..2078492	-	1167	WP_002292137.1	radical SAM family heme chaperone HemW	-
K9N64_RS12530 (K9N64_12510)	2078508..2078735	-	228	Protein_1947	hypothetical protein	-
K9N64_RS12535 (K9N64_12515)	2078987..2079253	-	267	WP_002292138.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9N64_RS12540 (K9N64_12520)	2079250..2079480	-	231	WP_002292139.1	DUF6290 family protein	Antitoxin
K9N64_RS12545 (K9N64_12525)	2079972..2080637	-	666	WP_002292140.1	hypothetical protein	-
K9N64_RS12550 (K9N64_12530)	2080774..2081733	-	960	WP_000222573.1	IS30 family transposase	-
K9N64_RS12555 (K9N64_12535)	2081880..2082134	+	255	WP_002316334.1	hypothetical protein	-
K9N64_RS12560 (K9N64_12540)	2082158..2082577	+	420	WP_225479451.1	toxin	-
K9N64_RS12565 (K9N64_12545)	2082809..2083567	-	759	WP_167779246.1	restriction endonuclease	-
K9N64_RS12570	2083759..2084013	+	255	WP_087063755.1	toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2080774..2081733	959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-72)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10556.21 Da
• Isoelectric Point: 10.3338

>T218368 WP_002292138.1 NZ_CP084248:c2079253-2078987 [Enterococcus lactis]
MTYRLEFTPEIQKQLRKMDKHQATLIVRWLYQHIDGIDDPRKFGKGLTANRSGQWRYRIGKYRVLVEIEDNQIVVIAIQV
GHRKNVYD

Nucleotide

• Length: 267 bp

>T218368 NZ_CP115887:c1935899-1935747 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAAGTAAAGAAATGGAACAAATTTCCGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8883.02 Da
• Isoelectric Point: 4.1943

>AT218368 WP_002292139.1 NZ_CP084248:c2079480-2079250 [Enterococcus lactis]
MAMITVRVSDAEKEWLSYMAEFYGITLSELLKTYSMEQLEDEYDRQTAEIAHKHWVESGKTTVSMEEILREFGGLE

Nucleotide

• Length: 231 bp

>AT218368 NZ_CP115887:c1936523-1935924 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA