Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 45566..46167 Replicon plasmid pY3-1
Accession NZ_CP084247
Organism Enterococcus lactis strain DH9003

Toxin (Protein)


Gene name mazF Uniprot ID A0A0V7YM55
Locus tag K9N64_RS01030 Protein ID WP_002330364.1
Coordinates 45823..46167 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B3Q8V0
Locus tag K9N64_RS01025 Protein ID WP_002318881.1
Coordinates 45566..45829 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9N64_RS00990 (K9N64_00990) 40911..41870 - 960 WP_002334843.1 IS30-like element IS6770 family transposase -
K9N64_RS00995 (K9N64_00995) 42064..42222 - 159 Protein_43 IS3 family transposase -
K9N64_RS01000 (K9N64_01000) 42274..42435 - 162 Protein_44 IS256 family transposase -
K9N64_RS01005 (K9N64_01005) 42513..44060 - 1548 WP_002296127.1 IS66 family transposase -
K9N64_RS01010 (K9N64_01010) 44162..44515 - 354 WP_002287659.1 IS66 family insertion sequence element accessory protein TnpB -
K9N64_RS01015 (K9N64_01015) 44505..44699 - 195 WP_002285758.1 hypothetical protein -
K9N64_RS01020 (K9N64_01020) 44840..45379 - 540 Protein_48 IS30-like element IS6770 family transposase -
K9N64_RS01025 (K9N64_01025) 45566..45829 + 264 WP_002318881.1 PbsX family transcriptional regulator Antitoxin
K9N64_RS01030 (K9N64_01030) 45823..46167 + 345 WP_002330364.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K9N64_RS01035 (K9N64_01035) 46282..46587 + 306 Protein_51 IS982 family transposase -
K9N64_RS01040 (K9N64_01040) 46662..47344 + 683 WP_162897317.1 IS6-like element IS1216 family transposase -
K9N64_RS01045 (K9N64_01045) 47399..47608 - 210 Protein_53 recombinase family protein -
K9N64_RS01050 (K9N64_01050) 47982..48248 - 267 WP_002291092.1 hypothetical protein -
K9N64_RS01055 (K9N64_01055) 48253..48417 - 165 WP_025477461.1 leucocin A/sakacin P family class II bacteriocin -
K9N64_RS01060 48510..48707 - 198 WP_002339781.1 hypothetical protein -
K9N64_RS01065 (K9N64_01060) 48998..49603 + 606 Protein_57 recombinase family protein -
K9N64_RS01070 (K9N64_01065) 49600..50808 + 1209 WP_225479028.1 putative phage abortive infection protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ClpL - 1..292841 292841
- inside IScluster/Tn - - 40911..47344 6433


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13024.11 Da        Isoelectric Point: 8.8971

>T218366 WP_002330364.1 NZ_CP084247:45823-46167 [Enterococcus lactis]
MVDVPRQGDILLLNTAPRSGHEQTGRRPYIVLSHDIIADTSNVVIVAPITTTSRNYPLYIEITENHKMKTRGKVMLDQIT
TIDYEARNCKFLEKASEKLIEELLTKIRAVFQKV

Download         Length: 345 bp

>T218366 NZ_CP115887:c1562599-1562354 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10071.69 Da        Isoelectric Point: 5.2300

>AT218366 WP_002318881.1 NZ_CP084247:45566-45829 [Enterococcus lactis]
MEMIPIKKWGNSNGLRLPKNLMEFLELKTDDRVSIKSEDVNGKRRLIIEPAISKEEVTIEELFKDYTEGKVNIKIQDLGS
PVGRELW

Download         Length: 264 bp

>AT218366 NZ_CP115887:1562020-1562385 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V7YM55

Antitoxin

Source ID Structure
AlphaFold DB A0A6B3Q8V0

References