Detailed information of TA system TA218364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 99703..100439
• Replicon: plasmid pKPT877-hybrid
• Accession: NZ_CP084242
• Organism: Klebsiella pneumoniae strain T877

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: LDH71_RS26140
• Protein ID: WP_004187044.1
• Coordinates: 99957..100439 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LDH71_RS26135
• Protein ID: WP_003026799.1
• Coordinates: 99703..99969 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDH71_RS26120 (LDH71_26155)	95175..96569	+	1395	WP_032439652.1	cytosine permease	-
LDH71_RS26125 (LDH71_26160)	96581..97789	+	1209	WP_032448288.1	imidazolonepropionase	-
LDH71_RS26130 (LDH71_26165)	97782..98591	+	810	WP_023329017.1	N-formylglutamate deformylase	-
LDH71_RS26135 (LDH71_26170)	99703..99969	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LDH71_RS26140 (LDH71_26175)	99957..100439	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
LDH71_RS26145 (LDH71_26180)	100646..101992	+	1347	WP_077254728.1	ISNCY family transposase	-
LDH71_RS26150 (LDH71_26185)	102041..102439	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
LDH71_RS26155 (LDH71_26190)	102587..102697	-	111	Protein_102	IS3 family transposase	-
LDH71_RS26160 (LDH71_26195)	102972..103908	+	937	Protein_103	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / tet(X4) / lnu(F) / aadA2 / blaOXA-181	iutA / iucD / iucC / iucB / iucA	1..293653	293653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T218364 WP_004187044.1 NZ_CP084242:99957-100439 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T218364 NZ_CP115887:c829911-829723 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTTACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT218364 WP_003026799.1 NZ_CP084242:99703-99969 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT218364 NZ_CP115887:c830200-829940 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66