Detailed information of TA system TA218363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 54795..55438
• Replicon: plasmid pKPT877-hybrid
• Accession: NZ_CP084242
• Organism: Klebsiella pneumoniae strain T877

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LDH71_RS25920
• Protein ID: WP_016236302.1
• Coordinates: 55022..55438 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: LDH71_RS25915
• Protein ID: WP_001261282.1
• Coordinates: 54795..55025 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDH71_RS25885 (LDH71_25920)	49804..50979	+	1176	Protein_48	IS3 family transposase	-
LDH71_RS25890 (LDH71_25925)	51361..52146	-	786	WP_050485696.1	site-specific integrase	-
LDH71_RS25895 (LDH71_25930)	52192..52713	-	522	WP_032448294.1	hypothetical protein	-
LDH71_RS25900 (LDH71_25935)	52771..53163	-	393	WP_074422814.1	hypothetical protein	-
LDH71_RS25905 (LDH71_25940)	53272..54216	-	945	WP_032448293.1	hypothetical protein	-
LDH71_RS25910 (LDH71_25945)	54416..54838	-	423	WP_050485703.1	hypothetical protein	-
LDH71_RS25915 (LDH71_25950)	54795..55025	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LDH71_RS25920 (LDH71_25955)	55022..55438	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
LDH71_RS25925 (LDH71_25960)	55512..57074	+	1563	WP_004206609.1	AAA family ATPase	-
LDH71_RS25930 (LDH71_25965)	57059..58081	+	1023	WP_032439672.1	helicase UvrD	-
LDH71_RS25935 (LDH71_25970)	58626..59534	+	909	WP_137877090.1	HNH endonuclease	-
LDH71_RS25940 (LDH71_25975)	59720..60070	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / tet(X4) / lnu(F) / aadA2 / blaOXA-181	iutA / iucD / iucC / iucB / iucA	1..293653	293653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T218363 WP_016236302.1 NZ_CP084242:55022-55438 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T218363 NZ_CP115874:c4568259-4567888 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT218363 WP_001261282.1 NZ_CP084242:54795-55025 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT218363 NZ_CP115874:c4568504-4568262 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3