Detailed information of TA system TA218362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5191494..5192119
• Replicon: chromosome
• Accession: NZ_CP084241
• Organism: Klebsiella pneumoniae strain T877

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: LDH71_RS25275
• Protein ID: WP_002882817.1
• Coordinates: 5191494..5191877 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LDH71_RS25280
• Protein ID: WP_162550505.1
• Coordinates: 5191877..5192119 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDH71_RS25260	5188860..5189762	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
LDH71_RS25265	5189759..5190394	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
LDH71_RS25270	5190391..5191320	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
LDH71_RS25275	5191494..5191877	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
LDH71_RS25280	5191877..5192119	-	243	WP_162550505.1	CopG family transcriptional regulator	Antitoxin
LDH71_RS25285	5192324..5193241	+	918	WP_032418237.1	alpha/beta hydrolase	-
LDH71_RS25290	5193255..5194196	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
LDH71_RS25295	5194241..5194678	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
LDH71_RS25300	5194675..5195535	-	861	WP_004146232.1	virulence factor BrkB family protein	-
LDH71_RS25305	5195529..5196128	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T218362 WP_002882817.1 NZ_CP084241:c5191877-5191494 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T218362 NZ_CP115874:c4444810-4444625 [Enterobacter hormaechei subsp. steigerwaltii]
GTGAAAAGTGCGGATATCATTGCTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGAAGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTAACCGTTCCGCATCCTAAAAAGGACATTAAACCGGGCACTCTCGCACAGATCT
GGCGACAGGCGGGCATTAAACACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9524.80 Da
• Isoelectric Point: 4.2500

>AT218362 WP_162550505.1 NZ_CP084241:c5192119-5191877 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDLAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT218362 NZ_CP115874:c4444610-4444206 [Enterobacter hormaechei subsp. steigerwaltii]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGCGGTTTCTTCCCCGACGTTCCGGGATGTTTTTT
TGCGGGGGATTCACTTGATGACGCTTTTCAGGATGCACGTGATGCGCTCACGGCGCACTTCGAAGCGCTGTTTGAAATGG
ATGAAGCATTGCCTCTCCCGGGTAGTGTCGAAGTGCACCTTGAAAATCAACCTGACGACTTCACAGGCGGGCAATGGTTG
CTGGTGGATATTAATATGAAGCAATTCGACGGCAGAGCCGAACGCATCAACATCACGATGCCTCGACGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCTCAATTCGGCAATCGAAGCGCATTTCTGGCCGAGGCAGCACGTCGCGTGCTGC
CGTAG

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8