Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4592388..4593198 Replicon chromosome
Accession NZ_CP084241
Organism Klebsiella pneumoniae strain T877

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LDH71_RS22455 Protein ID WP_004178461.1
Coordinates 4592388..4592921 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LDH71_RS22460 Protein ID WP_002887278.1
Coordinates 4592932..4593198 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDH71_RS22450 (4591219) 4591219..4592340 + 1122 WP_004214138.1 cupin domain-containing protein -
LDH71_RS22455 (4592388) 4592388..4592921 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LDH71_RS22460 (4592932) 4592932..4593198 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LDH71_RS22465 (4593301) 4593301..4594734 - 1434 WP_032439581.1 Cu(+)/Ag(+) sensor histidine kinase -
LDH71_RS22470 (4594724) 4594724..4595407 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LDH71_RS22475 (4595579) 4595579..4596964 + 1386 WP_032439580.1 efflux transporter outer membrane subunit -
LDH71_RS22480 (4596982) 4596982..4597326 + 345 WP_032418103.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T218361 WP_004178461.1 NZ_CP084241:c4592921-4592388 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T218361 NZ_CP115874:c3780834-3780424 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGTTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT218361 WP_002887278.1 NZ_CP084241:c4593198-4592932 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT218361 NZ_CP115874:c3781081-3780815 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References