Detailed information of TA system TA218332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 11345..12144
• Replicon: plasmid pArsBee4
• Accession: NZ_CP084226
• Organism: Arsenophonus apicola strain ArsBeeUS

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: LDL57_RS16990
• Protein ID: WP_225507760.1
• Coordinates: 11620..12144 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: LDL57_RS16985
• Protein ID: WP_225507762.1
• Coordinates: 11345..11623 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL57_RS17875	7827..8120	-	294	WP_255653937.1	DUF6012 family protein	-
LDL57_RS17880	8177..8392	-	216	WP_255653935.1	DUF6012 family protein	-
LDL57_RS16965 (LDL57_16965)	8413..8694	-	282	WP_225507931.1	hypothetical protein	-
LDL57_RS16970 (LDL57_16970)	8775..9086	-	312	WP_180559739.1	TrbM/KikA/MpfK family conjugal transfer protein	-
LDL57_RS16975 (LDL57_16975)	9164..9691	+	528	WP_180559725.1	lipocalin family protein	-
LDL57_RS16980 (LDL57_16980)	9698..10957	-	1260	WP_225507933.1	shufflon system plasmid conjugative transfer pilus tip adhesin PilV	-
LDL57_RS16985 (LDL57_16985)	11345..11623	+	279	WP_225507762.1	DUF1778 domain-containing protein	Antitoxin
LDL57_RS16990 (LDL57_16990)	11620..12144	+	525	WP_225507760.1	GNAT family N-acetyltransferase	Toxin
LDL57_RS16995 (LDL57_16995)	12187..12285	+	99	Protein_17	helix-turn-helix domain-containing protein	-
LDL57_RS17000 (LDL57_17000)	12418..12675	-	258	WP_225507935.1	bacteriocin immunity protein	-
LDL57_RS17005 (LDL57_17005)	12675..15335	-	2661	WP_225507937.1	S-type pyocin domain-containing protein	-
LDL57_RS17010 (LDL57_17010)	15393..15908	-	516	WP_225507729.1	A24 family peptidase	-
LDL57_RS17015 (LDL57_17015)	16055..16489	-	435	WP_225507955.1	lytic transglycosylase domain-containing protein	-
LDL57_RS17020 (LDL57_17020)	16648..16815	+	168	WP_225507939.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..50623	50623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (3-88)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19074.08 Da
• Isoelectric Point: 9.3469

>T218332 WP_225507760.1 NZ_CP084226:11620-12144 [Arsenophonus apicola]
MTLPIWHEEPIGKKHNRGDFNCGNEVLNQFLYRHARQNHEKGGAKTYLAVNEHNEKILGYYCLSPASIAYEDVPEVIKRG
LARHDVPVFRLGRLAVDFSVQGLGLGGQLLLAAGRRCLLVATQVGGVALVIDAKNERVAQWYASYGAIPLLDAKSTLMLP
FKTLHAALSAAGKL

Nucleotide

• Length: 525 bp

>T218332 NZ_CP115837:1806005-1806337 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10415.10 Da
• Isoelectric Point: 8.8278

>AT218332 WP_225507762.1 NZ_CP084226:11345-11623 [Arsenophonus apicola]
MPQIPVENNDRMSLRIASEEKSLLIRAAALQHTNLTEFVLRNVLSVARKIIDENERLNLTRRDSLHVMELLDNPPAPNDK
LMAAAFALPKKS

Nucleotide

• Length: 279 bp

>AT218332 NZ_CP115837:1805747-1806004 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA