Detailed information of TA system TA218331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 30355..31154
• Replicon: plasmid pArsBee2
• Accession: NZ_CP084224
• Organism: Arsenophonus apicola strain ArsBeeUS

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: LDL57_RS16335
• Protein ID: WP_225507760.1
• Coordinates: 30355..30879 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: LDL57_RS16340
• Protein ID: WP_225507762.1
• Coordinates: 30876..31154 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL57_RS16310 (LDL57_16310)	26336..27187	+	852	WP_225507856.1	Vmh family MBL fold metallo-hydrolase	-
LDL57_RS16315 (LDL57_16315)	27415..27696	-	282	Protein_34	DUF1440 domain-containing protein	-
LDL57_RS16320 (LDL57_16320)	27709..28863	-	1155	Protein_35	IS3 family transposase	-
LDL57_RS16325 (LDL57_16325)	28907..30073	-	1167	WP_225507858.1	alpha-hydroxy-acid oxidizing protein	-
LDL57_RS16330 (LDL57_16330)	30091..30315	-	225	WP_225507758.1	hypothetical protein	-
LDL57_RS16335 (LDL57_16335)	30355..30879	-	525	WP_225507760.1	GNAT family N-acetyltransferase	Toxin
LDL57_RS16340 (LDL57_16340)	30876..31154	-	279	WP_225507762.1	DUF1778 domain-containing protein	Antitoxin
LDL57_RS16345 (LDL57_16345)	31541..32818	+	1278	WP_225507764.1	shufflon system plasmid conjugative transfer pilus tip adhesin PilV	-
LDL57_RS16350 (LDL57_16350)	32806..33331	-	526	Protein_41	lipocalin family protein	-
LDL57_RS16355 (LDL57_16355)	33409..33693	+	285	WP_225507766.1	hypothetical protein	-
LDL57_RS16360 (LDL57_16360)	33723..34082	+	360	WP_180559738.1	hypothetical protein	-
LDL57_RS17870	34103..34483	+	381	WP_255653930.1	DUF6012 family protein	-
LDL57_RS16370 (LDL57_16370)	34603..35028	+	426	WP_225507870.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..59938	59938
	-	flank	IS/Tn	-	-	27709..28113	404

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (3-88)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19074.08 Da
• Isoelectric Point: 9.3469

>T218331 WP_225507760.1 NZ_CP084224:c30879-30355 [Arsenophonus apicola]
MTLPIWHEEPIGKKHNRGDFNCGNEVLNQFLYRHARQNHEKGGAKTYLAVNEHNEKILGYYCLSPASIAYEDVPEVIKRG
LARHDVPVFRLGRLAVDFSVQGLGLGGQLLLAAGRRCLLVATQVGGVALVIDAKNERVAQWYASYGAIPLLDAKSTLMLP
FKTLHAALSAAGKL

Nucleotide

• Length: 525 bp

>T218331 NZ_CP115837:1458596-1459078 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10415.10 Da
• Isoelectric Point: 8.8278

>AT218331 WP_225507762.1 NZ_CP084224:c31154-30876 [Arsenophonus apicola]
MPQIPVENNDRMSLRIASEEKSLLIRAAALQHTNLTEFVLRNVLSVARKIIDENERLNLTRRDSLHVMELLDNPPAPNDK
LMAAAFALPKKS

Nucleotide

• Length: 279 bp

>AT218331 NZ_CP115837:1458342-1458608 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA