Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 8987..9660 Replicon plasmid p3
Accession NZ_CP084215
Organism Pseudomonas amygdali strain 35-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A2V0QPW6
Locus tag LCH33_RS29495 Protein ID WP_017684243.1
Coordinates 9241..9660 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag LCH33_RS29490 Protein ID WP_003381346.1
Coordinates 8987..9244 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH33_RS29460 (LCH33_005865) 4101..5015 + 915 WP_054071525.1 DUF4868 domain-containing protein -
LCH33_RS29465 (LCH33_005866) 5076..6005 - 930 WP_054987848.1 DUF4928 family protein -
LCH33_RS29470 (LCH33_005867) 6005..7153 - 1149 WP_225231728.1 DNA cytosine methyltransferase -
LCH33_RS29475 (LCH33_005868) 7580..7942 + 363 WP_007247606.1 hypothetical protein -
LCH33_RS29480 (LCH33_005869) 7945..8172 + 228 WP_004642455.1 hypothetical protein -
LCH33_RS29485 (LCH33_005870) 8248..8880 - 633 WP_054987853.1 recombinase family protein -
LCH33_RS29490 (LCH33_005871) 8987..9244 + 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
LCH33_RS29495 (LCH33_005872) 9241..9660 + 420 WP_017684243.1 type II toxin-antitoxin system VapC family toxin Toxin
LCH33_RS29500 (LCH33_005873) 9701..10310 + 610 Protein_12 recombinase family protein -
LCH33_RS29505 (LCH33_005874) 10609..11544 + 936 WP_225231729.1 AvrD family protein -
LCH33_RS29510 (LCH33_005875) 11885..12532 + 648 WP_005746177.1 histidine phosphatase family protein -
LCH33_RS29515 (LCH33_005876) 12771..13598 + 828 WP_054987851.1 TauD/TfdA family dioxygenase -
LCH33_RS29520 (LCH33_005877) 13616..14650 + 1035 WP_054987854.1 sulfotransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..54338 54338


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15152.46 Da        Isoelectric Point: 4.7010

>T218305 WP_017684243.1 NZ_CP084215:9241-9660 [Pseudomonas amygdali]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAIETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Download         Length: 420 bp

>T218305 NZ_CP115820:2529250-2529834 [Pseudomonas tohonis]
ATGAACATCGACACCATCAGGGCGCGGCTCGACTTCCTGCGTGAGGCGGAGCGCCTCAAGGATGTGCTGCGCAGCGCCCA
CACCTCTTCCGGGCGGGCGGAAAGCACCGCCGAGCACAGCTGGCGCCTGTGCCTGATGGCCATCACCTTCGCCGACGAGC
TGGCCGGGCTCGACCTGCTGCGGGTGCTGAAGATGTGCGTGGTGCACGACCTGGGCGAGGCCATCCATGGCGACATCCCC
GCCGTCGAGCAGGGCGGCCACCCCGACAAGGGGGCCCGGGAGCGCGAGGACTTGCTGACCCTCACCCGCACCCTGGACGA
TGGGCTGCGCGGGGAGATCCTCGCGCTGTGGGACGACTACGAGAACGCCGCCTCCCCCGAGGCGCTGGCGGTCAAGGCGC
TGGACAAGCTGGAGACCCTGCTGCAGCACACCCAGGGCGCCAACCCGGAGGACTTCGACTACGCCTTCAACCTCGGCTAC
GGGCGCCGCTACACCAGCGGCGAACCGCTGTTCGCGGCCATCCGTGCACAGCTGGACGAGGACACGAGGCGGCGGATGGA
GGCGCAGGCGAGCGCGGCTCGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT218305 WP_003381346.1 NZ_CP084215:8987-9244 [Pseudomonas amygdali]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT218305 NZ_CP115820:2528581-2529138 [Pseudomonas tohonis]
ATGCACGACAGCCTCCCGAAACCGGTCTCGCTCGGCCTCGCGCTGCGCCGTTGGCGCCTGTTGCACCGGGTGAAGCAGGC
CCACGCCGCGCAGCTGTTCGGCGTCGCCCAGTCGACCATCTCGCGCTGGGAAAGCGGGGCGCAGGCGATGGAGCCGGCCG
AGCGCGCGCGGGTGGAGCAGGTGCTTGGCGCCCGGCTGGAGGCGGCGGCCGACCAGGCCCTGGCGCGGCTGGTGGGCGAC
AGCCCGCGCGCGGTGCACCTGGTCTGCGACCTGAGCCATCGCCTGCTGGCCTGTTCCGCCCTGCGCGCCGCCGAATTCAG
CGTGCCCCTCTCCGAGCTGCTGGGCCGCTCGCTGTGGCGCTACGCGACGCCGGAGATCGTGCGCAAGGAAGCCGCGCTGG
ACGCCCTGGGCTGGCGCGACGTGCTGATGCCGCCGGCGCTGGAGTTCGGCAGCGGCAGCAACGAATCCGCCATCGTGCCG
ATCCGCCAGGGCCTGTGCCGCTGGACGCGCATGACGCTTTCCGACGGCACCGCCGCGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPW6

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References