Detailed information of TA system TA218304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 17258..17735
• Replicon: plasmid p1
• Accession: NZ_CP084213
• Organism: Pseudomonas amygdali strain 35-1

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A0P9I0A0
• Locus tag: LCH33_RS28740
• Protein ID: WP_005730593.1
• Coordinates: 17469..17735 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A3R8W5Z2
• Locus tag: LCH33_RS28735
• Protein ID: WP_005730594.1
• Coordinates: 17258..17485 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH33_RS28700 (LCH33_005714)	12962..13357	+	396	WP_102886351.1	hypothetical protein	-
LCH33_RS28705 (LCH33_005715)	13379..13939	+	561	WP_180988335.1	single-stranded DNA-binding protein	-
LCH33_RS28710 (LCH33_005716)	14130..14957	+	828	WP_102886353.1	DUF932 domain-containing protein	-
LCH33_RS28715 (LCH33_005717)	15016..15438	+	423	WP_102886354.1	hypothetical protein	-
LCH33_RS28720 (LCH33_005718)	15471..15776	+	306	WP_225231715.1	TrfB-related DNA-binding protein	-
LCH33_RS28725 (LCH33_005719)	15798..16082	+	285	WP_102886355.1	hypothetical protein	-
LCH33_RS28730 (LCH33_005720)	16195..16797	+	603	WP_102886356.1	hypothetical protein	-
LCH33_RS28735 (LCH33_005721)	17258..17485	+	228	WP_005730594.1	hypothetical protein	Antitoxin
LCH33_RS28740 (LCH33_005722)	17469..17735	+	267	WP_005730593.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCH33_RS28745 (LCH33_005723)	17858..19135	-	1278	WP_225231716.1	LLM class flavin-dependent oxidoreductase	-
LCH33_RS28750 (LCH33_005724)	19169..19933	-	765	WP_102886303.1	ABC transporter ATP-binding protein	-
LCH33_RS28755 (LCH33_005725)	19958..20746	-	789	WP_102886304.1	ABC transporter permease	-
LCH33_RS28760 (LCH33_005726)	20743..21648	-	906	WP_102886305.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib	-	1..76741	76741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10201.73 Da
• Isoelectric Point: 10.2748

>T218304 WP_005730593.1 NZ_CP084213:17469-17735 [Pseudomonas amygdali]
MAWTIDYTDTAKTQLRKLDKQSAKRILDFMDERVSSRDDPRTTGKALTGPLGGLWRYRVGDFRVICEIQDGALRILVVQL
GNRREVYR

Nucleotide

• Length: 267 bp

>T218304 NZ_CP115820:1805609-1805902 [Pseudomonas tohonis]
ATGATGGAAGAGCTGTACGGCTACGATCCGGCCGAAGCGCTGGGCAGCCCGGAGGCCATTGCAGTATTCGTGGCCGATGC
GTTGGAGACCGGCGATGCGGCTTATATCGCCAAGGCGATGGGAGTGGTCGCTCGGGCGAAGGGGATGACCGAGCTGGCGC
GGGAGACCGGTCTGTCCCGGGAGCAGCTGTATCGTTCTTTCAGCGACCAGGGGAACCCGACCCTACGGACCATGCTCGCC
GTCATGCGTGCCCTTGGGCTGGACATGACTGCCAAGCCCCACAGTGCCGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8540.57 Da
• Isoelectric Point: 4.6135

>AT218304 WP_005730594.1 NZ_CP084213:17258-17485 [Pseudomonas amygdali]
MATSIRLAPETEQRLDFLATSTGRTKAYYLREIIDHGLTDLEDYYLAAEVLERVRKNTEVVHSAADVRKDLGLDD

Nucleotide

• Length: 228 bp

>AT218304 NZ_CP115820:1805313-1805612 [Pseudomonas tohonis]
ATGATTACCATCAGGCAGACCGCGACCTTCGCGGCCTGGGAGCGGAAGCTCAAGGACCGCAAAGCCCGAGCAGCCATTGC
CGCACGCATCTTCCGGCTGGCCAATGGCCTCCCCGGGGATGTCGCTCCGGTAGGGCAGGGCGTGAGCGAGATGCGGATTA
ATTACGGCCCCGGCTACCGCGTCTATTTCCAGCAGCGGGGCAATGAACTGGTGATCCTGCTCTGTGGCGGAGACAAATCC
AGCCAGGGCCGGGACATCGAGCAGGCCAGGCGCCTGGCGGCTGAGTGGAGGTCGCAATGA

Structures

Toxin

• AlphaFold DB: A0A0P9I0A0

Antitoxin

• AlphaFold DB: A0A3R8W5Z2