Detailed information of TA system TA218303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 8491..9089
• Replicon: plasmid p1
• Accession: NZ_CP084213
• Organism: Pseudomonas amygdali strain 35-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A6H1Q9F7
• Locus tag: LCH33_RS28670
• Protein ID: WP_028692294.1
• Coordinates: 8787..9089 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LCH33_RS28665
• Protein ID: WP_028692295.1
• Coordinates: 8491..8790 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH33_RS28635 (LCH33_005701)	3593..4780	-	1188	WP_102886348.1	Y-family DNA polymerase	-
LCH33_RS28640 (LCH33_005702)	4933..6438	+	1506	WP_100226550.1	IS21 family transposase	-
LCH33_RS28645 (LCH33_005703)	6428..7171	+	744	WP_029240427.1	IS21-like element helper ATPase IstB	-
LCH33_RS28650 (LCH33_005704)	7227..7652	-	426	Protein_8	IS110 family transposase	-
LCH33_RS28655 (LCH33_005705)	7726..8229	+	504	Protein_9	IS5 family transposase	-
LCH33_RS28660 (LCH33_005706)	8250..8471	-	222	WP_061305035.1	hypothetical protein	-
LCH33_RS28665 (LCH33_005707)	8491..8790	-	300	WP_028692295.1	putative addiction module antidote protein	Antitoxin
LCH33_RS28670 (LCH33_005708)	8787..9089	-	303	WP_028692294.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCH33_RS28675 (LCH33_005709)	9291..9533	+	243	WP_028692289.1	hypothetical protein	-
LCH33_RS28680 (LCH33_005710)	9585..11267	+	1683	WP_102886349.1	DNA cytosine methyltransferase	-
LCH33_RS28685 (LCH33_005711)	11354..11575	+	222	WP_028692287.1	hypothetical protein	-
LCH33_RS28690 (LCH33_005712)	11579..12019	+	441	WP_102886350.1	hypothetical protein	-
LCH33_RS28695 (LCH33_005713)	12031..12294	+	264	WP_061305040.1	hypothetical protein	-
LCH33_RS28700 (LCH33_005714)	12962..13357	+	396	WP_102886351.1	hypothetical protein	-
LCH33_RS28705 (LCH33_005715)	13379..13939	+	561	WP_180988335.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib	-	1..76741	76741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (7-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11277.85 Da
• Isoelectric Point: 10.3903

>T218303 WP_028692294.1 NZ_CP084213:c9089-8787 [Pseudomonas amygdali]
MKTIKQTATYRNWERKLRDKQAKAIIAARVFRVAHGLLGDVQPVGQGISELRIHHGPGYRVYFQQRGNQLVLLLCGGDKS
SQARDIETAKSLASQWSDDE

Nucleotide

• Length: 303 bp

>T218303 NZ_CP115820:1805313-1805612 [Pseudomonas tohonis]
ATGATTACCATCAGGCAGACCGCGACCTTCGCGGCCTGGGAGCGGAAGCTCAAGGACCGCAAAGCCCGAGCAGCCATTGC
CGCACGCATCTTCCGGCTGGCCAATGGCCTCCCCGGGGATGTCGCTCCGGTAGGGCAGGGCGTGAGCGAGATGCGGATTA
ATTACGGCCCCGGCTACCGCGTCTATTTCCAGCAGCGGGGCAATGAACTGGTGATCCTGCTCTGTGGCGGAGACAAATCC
AGCCAGGGCCGGGACATCGAGCAGGCCAGGCGCCTGGCGGCTGAGTGGAGGTCGCAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10825.27 Da
• Isoelectric Point: 5.1084

>AT218303 WP_028692295.1 NZ_CP084213:c8790-8491 [Pseudomonas amygdali]
MKEQFYDYDPADALTDAESIAVFMEDAFETGDAAYIAKCLGVVARAKGMTALANESGLSREHLYRSFSDKGNPTLKTLLA
VMRAVGLDLTTRKHVEPAH

Nucleotide

• Length: 300 bp

>AT218303 NZ_CP115820:1805609-1805902 [Pseudomonas tohonis]
ATGATGGAAGAGCTGTACGGCTACGATCCGGCCGAAGCGCTGGGCAGCCCGGAGGCCATTGCAGTATTCGTGGCCGATGC
GTTGGAGACCGGCGATGCGGCTTATATCGCCAAGGCGATGGGAGTGGTCGCTCGGGCGAAGGGGATGACCGAGCTGGCGC
GGGAGACCGGTCTGTCCCGGGAGCAGCTGTATCGTTCTTTCAGCGACCAGGGGAACCCGACCCTACGGACCATGCTCGCC
GTCATGCGTGCCCTTGGGCTGGACATGACTGCCAAGCCCCACAGTGCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A6H1Q9F7