Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA(toxin)
Location 6001688..6002327 Replicon chromosome
Accession NZ_CP084212
Organism Pseudomonas amygdali strain 35-1

Toxin (Protein)


Gene name hicA Uniprot ID A0A7Z6Y3C5
Locus tag LCH33_RS26810 Protein ID WP_005781617.1
Coordinates 6002145..6002327 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q48C83
Locus tag LCH33_RS26805 Protein ID WP_011169797.1
Coordinates 6001688..6002113 (-) Length 142 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH33_RS26790 (LCH33_005335) 5998154..5999476 + 1323 WP_057404930.1 murein hydrolase activator EnvC -
LCH33_RS26795 (LCH33_005336) 5999510..6000847 + 1338 WP_002551463.1 S41 family peptidase -
LCH33_RS26800 (LCH33_005337) 6000851..6001633 + 783 WP_004655075.1 divergent polysaccharide deacetylase family protein -
LCH33_RS26805 (LCH33_005338) 6001688..6002113 - 426 WP_011169797.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LCH33_RS26810 (LCH33_005339) 6002145..6002327 - 183 WP_005781617.1 type II toxin-antitoxin system HicA family toxin Toxin
LCH33_RS26815 (LCH33_005340) 6002649..6002889 + 241 Protein_5291 serine protease -
LCH33_RS26820 (LCH33_005341) 6002894..6003649 - 756 WP_004663214.1 ABC transporter substrate-binding protein -
LCH33_RS26825 (LCH33_005342) 6003835..6004605 - 771 WP_002551458.1 imidazole glycerol phosphate synthase subunit HisF -
LCH33_RS26830 (LCH33_005343) 6004625..6005362 - 738 WP_002551457.1 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase -
LCH33_RS26835 (LCH33_005344) 6005470..6005730 - 261 WP_002551456.1 DUF2164 domain-containing protein -
LCH33_RS26840 (LCH33_005345) 6005735..6006373 - 639 WP_005745046.1 imidazole glycerol phosphate synthase subunit HisH -
LCH33_RS26845 (LCH33_005346) 6006373..6006966 - 594 WP_002551454.1 imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-58)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6877.97 Da        Isoelectric Point: 10.6211

>T218302 WP_005781617.1 NZ_CP084212:c6002327-6002145 [Pseudomonas amygdali]
MKCNEFRRWLQAQGAEFRAAKGSHFKVYLNGKATIFADHGSKEMHEGLRKSIIKQLGLKD

Download         Length: 183 bp

>T218302 NZ_CP115819:823309-823590 [Cellulophaga omnivescoria]
ATAAGTAAACAATCGATTAAGGATTTAGATAAAATTTGGATTTATACGCTTAATAAATGGTTTAAAGAACAAGCTGACAG
ATATTACGACTTGATAATTACGGAAATTGAATTTATTGCTGACAACTTTATGACTGGAAAGTCAGCAGAACAAACTCGAA
AGAATTATCGAGTGACTAAAATAAAATCCCATTTGATTTTTTATAGAAAAGTGGAGAATGATATTGTGGAAATAGTTAGA
GTTTTACACCAAAGAATGGATATTAAAAAAGGACTGAAATAA

Antitoxin


Download         Length: 142 a.a.        Molecular weight: 15749.22 Da        Isoelectric Point: 5.2548

>AT218302 WP_011169797.1 NZ_CP084212:c6002113-6001688 [Pseudomonas amygdali]
MYQYPLDLHREPTGVWLSCPDIPEMSASGDTLPEALSEALDGMESALSLYVDQRRKIPQASVVPGHELLMRLPALTVAKI
MLWNSLLEEGVSRAELARRLGCTRQVVDRLVDFLHASKIEQVERALAELGRRITLSIERAA

Download         Length: 426 bp

>AT218302 NZ_CP115819:823050-823292 [Cellulophaga omnivescoria]
ATGGGAAAAAACACATCAATATCACTCGGAAATCATTTTGAGAATTTCATAAAAGAAGAAGTAAATTCTGGAAGATATGG
TTCTGTTAGCGAGGTAATTCGTTCTGCTTTACGCTTGTTAGAACGTGAAGAAAAAAAGGAAAGGGAACTAATTAAAGCTC
TCGAAGTTGGGGAAAATAGTGGATTTGATGAAAATTTTGACCCAAAACAACATTTGAAAGAATTACATCGTAAACACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z6Y3C5

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9WSP1

References