Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/upstrm_HI1419-dnstrm_HI1420
Location 5523579..5524174 Replicon chromosome
Accession NZ_CP084212
Organism Pseudomonas amygdali strain 35-1

Toxin (Protein)


Gene name relE Uniprot ID A0A3M3ZKH5
Locus tag LCH33_RS24550 Protein ID WP_005746690.1
Coordinates 5523579..5523881 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0P9S822
Locus tag LCH33_RS24555 Protein ID WP_005746689.1
Coordinates 5523884..5524174 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH33_RS24530 (LCH33_004882) 5518912..5520189 - 1278 WP_057404492.1 precorrin-3B synthase -
LCH33_RS24535 (LCH33_004883) 5520378..5521580 + 1203 WP_016982357.1 bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase -
LCH33_RS24540 (LCH33_004884) 5521580..5522692 + 1113 WP_054076830.1 cobalt-precorrin-5B (C(1))-methyltransferase -
LCH33_RS24545 (LCH33_004885) 5522689..5523429 + 741 WP_057404491.1 cobalt-precorrin-6A reductase -
LCH33_RS24550 (LCH33_004886) 5523579..5523881 + 303 WP_005746690.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LCH33_RS24555 (LCH33_004887) 5523884..5524174 + 291 WP_005746689.1 putative addiction module antidote protein Antitoxin
LCH33_RS24560 (LCH33_004888) 5524245..5524706 + 462 WP_002555389.1 copper chaperone PCu(A)C -
LCH33_RS24565 (LCH33_004889) 5524720..5525154 + 435 WP_016982355.1 DUF2946 domain-containing protein -
LCH33_RS24570 (LCH33_004890) 5525239..5526612 + 1374 WP_005746685.1 PepSY domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-86)

Antitoxin

Uncharacterized protein HI1420

(4-95)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11477.21 Da        Isoelectric Point: 10.6008

>T218301 WP_005746690.1 NZ_CP084212:5523579-5523881 [Pseudomonas amygdali]
MNRFEQTPAFAEWLRSLKDSIGRARILARIRAAELGNFGDCDALGQGVRELRIHHGPGYRVYFARRTGVVYLLLIAGDKS
SQKRDIKFARQLARELCERE

Download         Length: 303 bp

>T218301 NZ_CP115818:3648-3986 [Pseudomonas mendocina]
ATGACAACATTCGACGTACGCTTCACCGATTCCGCTTCTCAAAGCATCGAAGACCAGGTTCACCATCTGGCCGTTTACCA
GGGCACCGAAGCTGCCCTCGCGAGAATTGACTCGCTGGTAGATGTCATCACGGACAAACTCAAGTCCACCCCAATGGGAT
ACCCAGTCAGCCAGCAGGCGAGTGAGTTGGGGATCATCCATTACCGCGAACTCAATACGGACGGCTATCGAATCCTCTAT
GAGATTTTCGATACCGACATCGCGGTCGAGCTGGTTATAAGGCAGAAGCAGAGCGTCGAGCAGGCCCTGATCCGCTACTG
TTTGCTGAATCCGTTGTAG

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10500.87 Da        Isoelectric Point: 4.5720

>AT218301 WP_005746689.1 NZ_CP084212:5523884-5524174 [Pseudomonas amygdali]
MNNETTPFDTVDYLKTPEDMANYLDACLEEDSGDGLLVRAALNDIARAYGMTQIARDTGLGRESLYKALGANGNPEFTTI
MKVVRAMGIKLHASTR

Download         Length: 291 bp

>AT218301 NZ_CP115818:3387-3635 [Pseudomonas mendocina]
ATGAGGGTTGAAACGATTAGCTATTTGAAACGACACGCTGCCGATCTGGATCTCGATGAGCCCATGGTCGTCACGCAGAA
CGGTGTACCAGCCTATGTCGTGGAGTCTTACGCAGACCGTAAGCGCCGCGATGAAGCCATCGCTCTGGTGAAACTGCTCG
CAATTGGTTCTCGCGAGTACGCCCAAGGTAAGCATTGTTCTGCTGACGAACTGAAGGCCCGCCTGGCCAGGAGGTTTGCC
CAACACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M3ZKH5

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9S822

References