Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 55211..55854 Replicon plasmid pKP2648-KPCR
Accession NZ_CP084211
Organism Escherichia coli strain EC2648-R2

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LDB16_RS22900 Protein ID WP_001044770.1
Coordinates 55211..55627 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LDB16_RS22905 Protein ID WP_001261282.1
Coordinates 55624..55854 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB16_RS22880 (51314) 51314..51586 - 273 Protein_56 transposase -
LDB16_RS22890 (52568) 52568..53590 - 1023 WP_000361404.1 helicase UvrD -
LDB16_RS22895 (53575) 53575..55137 - 1563 WP_004206609.1 AAA family ATPase -
LDB16_RS22900 (55211) 55211..55627 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LDB16_RS22905 (55624) 55624..55854 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LDB16_RS22910 (55811) 55811..56272 + 462 WP_014343465.1 hypothetical protein -
LDB16_RS22915 (56433) 56433..57377 + 945 WP_011977810.1 hypothetical protein -
LDB16_RS22920 (57414) 57414..57806 + 393 WP_011977811.1 hypothetical protein -
LDB16_RS22925 (57864) 57864..58385 + 522 WP_013214008.1 hypothetical protein -
LDB16_RS22930 (58431) 58431..58634 + 204 WP_011977813.1 hypothetical protein -
LDB16_RS22935 (58664) 58664..59668 + 1005 WP_011977814.1 hypothetical protein -
LDB16_RS22940 (59852) 59852..60631 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / blaSHV-187 / blaKPC-2 - 1..102745 102745


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T218298 WP_001044770.1 NZ_CP084211:c55627-55211 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T218298 NZ_CP115784:c3033616-3033332 [Paracoccus aerodenitrificans]
ATGAGCTATGAGCTACGCTTCCGCAAGGAAGCCAAGAAAGAATGGGACAGTCTTGGCGCGACGATCCGTACCCAGTTCAA
AAAGAAGCTGGCCGAGAGGCTGGAAAGCCCGCATGTGCCAGCGTCCAAGCTTCATGGGTCCACGAACCGCTACAAGATCA
AATTGCGCTCGTCAGGCTACCGTCTGGTCTATGAGGTCAGGGACGCCGAGATCGTTGTCAGCGTTATTGCCGTTGGAAAG
CGGGAGCGAAGCGCCGTGTACAAGGCCGCAGCAAAGAGAGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT218298 WP_001261282.1 NZ_CP084211:c55854-55624 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT218298 NZ_CP115784:c3033854-3033606 [Paracoccus aerodenitrificans]
ATGGCAAATGTCATCCACGCTGGTGTGACCGCCAGTGTCACCGAGTTTAAGCGCAATCCTATGGGCACTATGGCCGCAGG
TAACGGTGCCGCTGTGGCTGTCCTGAACCGCAACCAACCTGCATTCTACTGCGTCCCAGCTGACGAGTATGAGGCGATGC
TGAACCTGATCGAGGATGCAGAGCTGAACGCCATCGCTGATGCACGATCTGACAGCCCTGAGATTGCGGTGTCTCTGGAT
GAGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References