Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4130595..4131092 Replicon chromosome
Accession NZ_CP084209
Organism Escherichia coli strain EC2648-R2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LDB16_RS20180 Protein ID WP_001173343.1
Coordinates 4130595..4130768 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LDB16_RS20185 Protein ID WP_000398619.1
Coordinates 4130796..4131092 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB16_RS20170 (4127265) 4127265..4128722 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LDB16_RS20175 (4128959) 4128959..4130476 + 1518 WP_001295074.1 lysine--tRNA ligase -
LDB16_RS20180 (4130595) 4130595..4130768 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LDB16_RS20185 (4130796) 4130796..4131092 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LDB16_RS20190 (4131319) 4131319..4131591 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
LDB16_RS20195 (4131603) 4131603..4131833 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LDB16_RS20200 (4132014) 4132014..4133645 + 1632 WP_001216477.1 sensor histidine kinase -
LDB16_RS20205 (4133642) 4133642..4134361 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T218297 WP_001173343.1 NZ_CP084209:c4130768-4130595 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T218297 NZ_CP115784:c624944-624636 [Paracoccus aerodenitrificans]
TTGAAGACACTTCTCATTGCCGAGCCGGCCGGGCGAGACCTTGAAGCCATCGTGGATTATATCGCGCGGGAAAACCCGGC
TGCGGCCGAGAATGTGTACAGGCAGATTGCCCGAACAGCCGCGAAGCTTCCGGAGTTTCCCGCACTCGGCCGTCCGGGGC
GCTTTTCCGGGACGCGGGAAATGAATGTGGCAGGGTCACCCTATCTCATTGTCTACGAGGTCAACGTCGAGGCAGTCACA
ATTCTGGCCGTCTTTCACACCTCCCGCAATCTCGCAGCGGCCCTTCGCGATCGTATGGAAGGCAGCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT218297 WP_000398619.1 NZ_CP084209:c4131092-4130796 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT218297 NZ_CP115784:c625192-624941 [Paracoccus aerodenitrificans]
ATGCCGCAGCGCGCCGAAACAGAACTCATGACCGTCCGCACAGCCAGGGTCTCGGAGATCGATGCCTTGGCCGCCGCGAT
GGATCGTTCTCGCAACTATGTGGTCAATCAGGCGATCGATCAGTTTCTCGAAGCCAACGCTTGGCAAATGGAACGGATCG
CGGCCGGGGTGGCCGCAGCGCGCGAGGGTAACGTCAGTTCTGCCGACGACGTCTTTGGCGCCATTGCCACCAAGCATGGC
TGGCAGGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References