Detailed information of TA system TA218295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3949623..3950437
• Replicon: chromosome
• Accession: NZ_CP084209
• Organism: Escherichia coli strain EC2648-R2

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: LDB16_RS19250
• Protein ID: WP_001054376.1
• Coordinates: 3949623..3949880 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: LDB16_RS19255
• Protein ID: WP_001309181.1
• Coordinates: 3949892..3950437 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB16_RS19225 (3944911)	3944911..3946017	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
LDB16_RS19230 (3946082)	3946082..3947062	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
LDB16_RS19235 (3947172)	3947172..3947377	+	206	Protein_3779	HNH endonuclease	-
LDB16_RS19240 (3947645)	3947645..3948885	-	1241	Protein_3780	helicase YjhR	-
LDB16_RS19245 (3949001)	3949001..3949132	+	132	WP_001309182.1	hypothetical protein	-
LDB16_RS19250 (3949623)	3949623..3949880	+	258	WP_001054376.1	YjhX family toxin	Toxin
LDB16_RS19255 (3949892)	3949892..3950437	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
LDB16_RS19260 (3950493)	3950493..3951239	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
LDB16_RS19265 (3951408)	3951408..3951626	+	219	Protein_3785	hypothetical protein	-
LDB16_RS19270 (3951664)	3951664..3951780	+	117	Protein_3786	VOC family protein	-
LDB16_RS19275 (3952025)	3952025..3953146	+	1122	WP_000010829.1	M42 family metallopeptidase	-
LDB16_RS19280 (3953143)	3953143..3953421	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
LDB16_RS19285 (3953433)	3953433..3954746	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	3938633..3958661	20028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T218295 WP_001054376.1 NZ_CP084209:3949623-3949880 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T218295 NZ_CP115782:15360-15641 [Paracoccus aerodenitrificans]
ATGATCCAAAGTACCAAGGGAAAGCTGATCCAGGACGTTCTGGCGAACAAGGCCAGCAAAGGCTTTCCCGCCGGTATCAT
GAAGGTTGCACGCCGCAAGCTGACCATGATCGACGCGGCGAGCAAGCTTGAGGATTTGCGGATGCCGCCTGCCAACAGGC
TTGAGGCGCTTCAAGGCGACCGCAAAGAGCAATACTCGATCCGGGTGAACGACCAATGGCGCTTTTGTTTCGTCTGGACC
GAAAGCGGCCCTGCTGATGTTGAATTTACGGACTACCACTGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT218295 WP_001309181.1 NZ_CP084209:3949892-3950437 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT218295 NZ_CP115782:15654-15944 [Paracoccus aerodenitrificans]
ATGGGACTGATGACCAACCCCTGCCACCCCGGCGAGATCCTCAAGCATGAATTTCTTGAGCCGCTCGGGGTTTCGGCGAT
TGGCCTGGCAAAAGCGATTGGCGTGCCACGCACGCGGGTTGAGCGACTGGTTAAGGAACAAACGGGGGTAACCCCTGACA
CGGCCCTGCGCCTGTCGAAAGCTCTTGGGACGACCCCTGAATTTTGGATCAATATGCAGGCGAATTTTGACATGGCCTCT
GCCCGTAAGAGCGTCGATGTGTCAGGCATTGAGCCGCTCATAGCGGCCTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8