Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3522205..3522884 | Replicon | chromosome |
| Accession | NZ_CP084209 | ||
| Organism | Escherichia coli strain EC2648-R2 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | LDB16_RS17155 | Protein ID | WP_000854672.1 |
| Coordinates | 3522205..3522546 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | LDB16_RS17160 | Protein ID | WP_000070395.1 |
| Coordinates | 3522567..3522884 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LDB16_RS17130 (3517791) | 3517791..3517883 | + | 93 | Protein_3367 | sigma factor-binding protein Crl | - |
| LDB16_RS17135 (3517922) | 3517922..3518977 | - | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| LDB16_RS17140 (3519265) | 3519265..3520368 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| LDB16_RS17145 (3520380) | 3520380..3521633 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| LDB16_RS17155 (3522205) | 3522205..3522546 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| LDB16_RS17160 (3522567) | 3522567..3522884 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| LDB16_RS17165 (3522903) | 3522903..3523124 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| LDB16_RS17170 (3523133) | 3523133..3523609 | - | 477 | WP_000811693.1 | RadC family protein | - |
| LDB16_RS17175 (3523625) | 3523625..3524083 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| LDB16_RS17180 (3524181) | 3524181..3524420 | - | 240 | WP_000194654.1 | DUF905 family protein | - |
| LDB16_RS17185 (3524497) | 3524497..3524964 | - | 468 | WP_001547765.1 | protein YkfB | - |
| LDB16_RS17190 (3524987) | 3524987..3525430 | - | 444 | WP_000824223.1 | lipoprotein YafY | - |
| LDB16_RS17195 (3525430) | 3525430..3525657 | - | 228 | WP_001548158.1 | protein YpjK | - |
| LDB16_RS17200 (3525653) | 3525653..3525844 | - | 192 | Protein_3380 | DeoR family transcriptional regulator | - |
| LDB16_RS17205 (3526061) | 3526061..3526882 | - | 822 | WP_000197389.1 | DUF932 domain-containing protein | - |
| LDB16_RS17210 (3526974) | 3526974..3527837 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 3517085..3555898 | 38813 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T218290 WP_000854672.1 NZ_CP084209:c3522546-3522205 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T218290 NZ_CP115777:66663-67070 [Paracoccus albus]
ATGCCAGGTAGGTTCTTTGACACGAACATCCTGCTTTATCTTTTCTCCGACGACCGAGCCAAGGCAGACATTGCCGAAGG
GCTATTGCGTGACGGCGGAACGGTCAGCGTGCAGGTGCTGAACGAGATCGCGAATGTCACGCAGCGGAAGTTCAAGATGA
GCTGGTCGCAGAGCGATGAATTTCTGCTCATGATCCGTGAATTTGTGACCGTTGAGCCATTGACCTATGAGACCCATGAT
CTGGGCATAGCCCTGGCGCGGAAACACACGCTGTCTGTCTATGACGCGATGATCGTCGCGGCCGGGTTGCTGGCAGGCTG
CGACACCGTGCTTTCCGAGGACATGCAGGACGGGTTCAGGGTGGCTGACAGGATTACGATCAGCAATCCATTCGCCGCAT
TAGAATGA
ATGCCAGGTAGGTTCTTTGACACGAACATCCTGCTTTATCTTTTCTCCGACGACCGAGCCAAGGCAGACATTGCCGAAGG
GCTATTGCGTGACGGCGGAACGGTCAGCGTGCAGGTGCTGAACGAGATCGCGAATGTCACGCAGCGGAAGTTCAAGATGA
GCTGGTCGCAGAGCGATGAATTTCTGCTCATGATCCGTGAATTTGTGACCGTTGAGCCATTGACCTATGAGACCCATGAT
CTGGGCATAGCCCTGGCGCGGAAACACACGCTGTCTGTCTATGACGCGATGATCGTCGCGGCCGGGTTGCTGGCAGGCTG
CGACACCGTGCTTTCCGAGGACATGCAGGACGGGTTCAGGGTGGCTGACAGGATTACGATCAGCAATCCATTCGCCGCAT
TAGAATGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT218290 WP_000070395.1 NZ_CP084209:c3522884-3522567 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT218290 NZ_CP115777:66443-66673 [Paracoccus albus]
ATGCAGATCGCGAAATGGGGTAATTCTCTGGCCGTCCGCCTGCCTGCGCGGATGGTCCAGGAACTCGGGCTTAAGGCGGG
CGACGAGATCGAAATCACGGCCACTGATGCCGGGCATTTTCAGGTGCAACGCCGCCGCGACCCCGAAGAAATTCTGGCAC
GCTTGCGCGAGTTTCGCGGGTTGATGCCCAAGGGCTATCGGTTCGACCGGGACGAGGCGAATGCCAGGTAG
ATGCAGATCGCGAAATGGGGTAATTCTCTGGCCGTCCGCCTGCCTGCGCGGATGGTCCAGGAACTCGGGCTTAAGGCGGG
CGACGAGATCGAAATCACGGCCACTGATGCCGGGCATTTTCAGGTGCAACGCCGCCGCGACCCCGAAGAAATTCTGGCAC
GCTTGCGCGAGTTTCGCGGGTTGATGCCCAAGGGCTATCGGTTCGACCGGGACGAGGCGAATGCCAGGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|