Detailed information of TA system TA218288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2369550..2370188
• Replicon: chromosome
• Accession: NZ_CP084209
• Organism: Escherichia coli strain EC2648-R2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LDB16_RS11470
• Protein ID: WP_000813794.1
• Coordinates: 2370012..2370188 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LDB16_RS11465
• Protein ID: WP_001270286.1
• Coordinates: 2369550..2369966 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB16_RS11445 (2364702)	2364702..2365643	-	942	WP_001251304.1	ABC transporter permease	-
LDB16_RS11450 (2365644)	2365644..2366657	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LDB16_RS11455 (2366675)	2366675..2367820	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LDB16_RS11460 (2368065)	2368065..2369471	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LDB16_RS11465 (2369550)	2369550..2369966	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LDB16_RS11470 (2370012)	2370012..2370188	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LDB16_RS11475 (2370410)	2370410..2370640	+	231	WP_000494244.1	YncJ family protein	-
LDB16_RS11480 (2370732)	2370732..2372693	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LDB16_RS11485 (2372766)	2372766..2373302	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LDB16_RS11490 (2373355)	2373355..2374569	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T218288 WP_000813794.1 NZ_CP084209:c2370188-2370012 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T218288 NZ_CP115776:c184272-183868 [Paracoccus albus]
ATGCTGATCCTTGACACGAATGTTATATCGGCGCTGCGCAGGCCAGAACGCGCGCCAGAGGTCGCGGCGTGGTTGAAGCG
GCAGGATGACAATGTGCTGTATCTGTCTGTCGTAACCCTCGGCGAGATTGAGCGCGGCATCGCGCGACAGGAACAGCAGG
ACCCGGCTTTTGCGCGCGATCTGCGCAACTGGCTGGGTCGGACGGTGCAGTTGTTCGGTGATCGCCTGCTGCCATTCGAT
GCGCAATCCGCGCGGATCTGGGGACAGTTGTCGGCGCGGTTGGGGCATGCGGGTGCCGATCTGCTGATTGCTGCAACAGC
ACTGCATCACGATGCGGTTGTCGTTACGCGCAATGTCTCGGACTTTGAGCCGACGGGTTGCCGGATCGAAAACCCCTTTG
CGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT218288 WP_001270286.1 NZ_CP084209:c2369966-2369550 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT218288 NZ_CP115776:c184520-184272 [Paracoccus albus]
ATGTGGAGTCTGCAGGACGCGAAGAACAAGTTTTCCGCTGTGGTGGATGCGGCGCTTGCGGGTGAGCCGCAAGAAGTCAC
GCGCCGGGGTAAGCCGGCTGTGGTCGTGCTTTCGGCATCCGAATATGCGCGGATATTGGCAGAGGCGCAGTCGAATCGTG
GCAGTTTCGCCAAGCATCTTCTGACCCAGCCACGCGGCGAGGTAGAGGCGGAACGGGCAGAGGCCCGGCCGCGCGACGTA
GCTTTCTGA

Structures

Toxin

• PDB: 6HPB