Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1802307..1803138 Replicon chromosome
Accession NZ_CP084209
Organism Escherichia coli strain EC2648-R2

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag LDB16_RS08580 Protein ID WP_000854814.1
Coordinates 1802307..1802681 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag LDB16_RS08585 Protein ID WP_001285584.1
Coordinates 1802770..1803138 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB16_RS08540 (1797703) 1797703..1798869 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
LDB16_RS08545 (1798988) 1798988..1799461 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
LDB16_RS08550 (1799659) 1799659..1800717 + 1059 WP_001200891.1 FUSC family protein -
LDB16_RS08555 (1800889) 1800889..1801218 + 330 WP_000450409.1 DUF496 family protein -
LDB16_RS08560 (1801319) 1801319..1801453 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
LDB16_RS08565 (1801573) 1801573..1801701 + 129 Protein_1682 transposase domain-containing protein -
LDB16_RS08570 (1801990) 1801990..1802070 - 81 Protein_1683 hypothetical protein -
LDB16_RS08575 (1802116) 1802116..1802310 - 195 WP_000988600.1 DUF5983 family protein -
LDB16_RS08580 (1802307) 1802307..1802681 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
LDB16_RS08585 (1802770) 1802770..1803138 - 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
LDB16_RS08590 (1803212) 1803212..1803433 - 222 WP_000692323.1 DUF987 domain-containing protein -
LDB16_RS08595 (1803496) 1803496..1803942 - 447 WP_000187523.1 RadC family protein -
LDB16_RS08600 (1803939) 1803939..1805471 - 1533 WP_001350525.1 protein YeeR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T218277 WP_000854814.1 NZ_CP084209:c1802681-1802307 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T218277 NZ_CP115738:510169-510519 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT218277 WP_001285584.1 NZ_CP084209:c1803138-1802770 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT218277 NZ_CP115738:509883-510164 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References