Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 113042..113685 Replicon plasmid pKPC-fusion-1
Accession NZ_CP084208
Organism Escherichia coli strain EC2648-R1-1

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LDB15_RS23070 Protein ID WP_001044770.1
Coordinates 113042..113458 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LDB15_RS23075 Protein ID WP_001261282.1
Coordinates 113455..113685 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB15_RS23050 (109145) 109145..109417 - 273 Protein_144 transposase -
LDB15_RS23060 (110399) 110399..111421 - 1023 WP_000361404.1 helicase UvrD -
LDB15_RS23065 (111406) 111406..112968 - 1563 WP_004206609.1 AAA family ATPase -
LDB15_RS23070 (113042) 113042..113458 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LDB15_RS23075 (113455) 113455..113685 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LDB15_RS23080 (113642) 113642..114103 + 462 WP_014343465.1 hypothetical protein -
LDB15_RS23085 (114264) 114264..115208 + 945 WP_011977810.1 hypothetical protein -
LDB15_RS23090 (115245) 115245..115637 + 393 WP_011977811.1 hypothetical protein -
LDB15_RS23095 (115695) 115695..116216 + 522 WP_013214008.1 hypothetical protein -
LDB15_RS23100 (116262) 116262..116465 + 204 WP_011977813.1 hypothetical protein -
LDB15_RS23105 (116495) 116495..117499 + 1005 WP_011977814.1 hypothetical protein -
LDB15_RS23110 (117683) 117683..118462 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / blaSHV-187 / blaKPC-2 - 1..139530 139530


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T218266 WP_001044770.1 NZ_CP084208:c113458-113042 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T218266 NZ_CP115714:802063-802473 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT218266 WP_001261282.1 NZ_CP084208:c113685-113455 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT218266 NZ_CP115714:801816-802082 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References