Detailed information of TA system TA218259

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 4331332..4332011
• Replicon: chromosome
• Accession: NZ_CP084207
• Organism: Escherichia coli strain EC2648-R1-1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: LDB15_RS20970
• Protein ID: WP_000854672.1
• Coordinates: 4331332..4331673 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: LDB15_RS20975
• Protein ID: WP_000070395.1
• Coordinates: 4331694..4332011 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB15_RS20945 (4326918)	4326918..4327010	+	93	Protein_4100	sigma factor-binding protein Crl	-
LDB15_RS20950 (4327049)	4327049..4328104	-	1056	WP_000749863.1	phosphoporin PhoE	-
LDB15_RS20955 (4328392)	4328392..4329495	+	1104	WP_001285288.1	glutamate 5-kinase	-
LDB15_RS20960 (4329507)	4329507..4330760	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
LDB15_RS20970 (4331332)	4331332..4331673	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
LDB15_RS20975 (4331694)	4331694..4332011	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
LDB15_RS20980 (4332030)	4332030..4332251	-	222	WP_000691994.1	DUF987 domain-containing protein	-
LDB15_RS20985 (4332260)	4332260..4332736	-	477	WP_000811693.1	RadC family protein	-
LDB15_RS20990 (4332752)	4332752..4333210	-	459	WP_000211838.1	antirestriction protein	-
LDB15_RS20995 (4333308)	4333308..4333547	-	240	WP_000194654.1	DUF905 family protein	-
LDB15_RS21000 (4333624)	4333624..4334091	-	468	WP_001547765.1	protein YkfB	-
LDB15_RS21005 (4334114)	4334114..4334557	-	444	WP_000824223.1	lipoprotein YafY	-
LDB15_RS21010 (4334557)	4334557..4334784	-	228	WP_001548158.1	protein YpjK	-
LDB15_RS21015 (4334780)	4334780..4334971	-	192	Protein_4113	DeoR family transcriptional regulator	-
LDB15_RS21020 (4335188)	4335188..4336009	-	822	WP_000197389.1	DUF932 domain-containing protein	-
LDB15_RS21025 (4336101)	4336101..4336964	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4326212..4365025	38813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T218259 WP_000854672.1 NZ_CP084207:c4331673-4331332 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T218259 NZ_CP115713:2449977-2450855 [Rhodopseudomonas palustris]
ATGGCTAAAGCACCAACCAATGTAGCGAAATCGGTCAAGGACCGGCTGCTCAATATCGCCCGTAAAGAAGGTCGCGCATT
CGACGTCCTGCTGGTGCGGTTCGCGCTCGAACGGCTACTTTATCGCCTCTCGATCTCAGCGCATAGCGACCGCTTCGTCC
TGAAAGGTGGCATGCTGGTCACGGTCTGGGTGGCCGACGACAATCGGGTGACGCGGGATGCGGACTTTCTCGGACATGGG
GACGCCGATCCTGACCGTCTGATCGCCGACTTCGGGGAGATCATGGCAATCGAGAGCGACGATGGCCTTGCCTTCGATAT
CGACGCGCTCACTGCGATGGTTATTCGAGAGGAGATGGAATACGGGGGCGTTCGCCTCAAAACCGCGGCCTATCTCGAAA
GGACGCACATTCCCGTTACAATCGACATCGGTTTTGGAGACGCTATGGCCGATGCGACGCAGCGGCTCGACTATCCGACA
TTGCTGGATCTTCCCGCCCCACAGGTTCGTTGCTATCCGCCCGCCACGGTGATCGCCGAGAAATTCCAGGCAATGGTGGA
GCTCGGCGTCCTCAACGGACGCATGAAGGACTATTACGACCTGTGGGCGATCCCACGCGCGATCGACATTTCCGCGGACG
ATCTGGATGCGGCCATCAGCGCGACGTTCGAGCGCCGGCGGACGCCGATCCCGAAAGTACGTCCACCAGGTCTCTCGGCC
GAAATGTTCGGCGACGAAGGGAAACAGCGGCAATGGCGTGCTTACGCCGCATCGCTCGAACTCGATGCTATTTCGCTGGA
ATCGATCATCGAAGCGGTTTGGGAGTTGGTCGGTCCGTCATGCGCGCGGATCGTAGCAAGAAGCGACAGTGAGGCATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT218259 WP_000070395.1 NZ_CP084207:c4332011-4331694 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT218259 NZ_CP115713:2449364-2449984 [Rhodopseudomonas palustris]
ATGCGCGCGACGCGAGATTCGCAAAAGGCCAAACTCATCAGCCTGCTTGAGACGCGCAGCATGATGCGCGCGCAGGACCT
GCGGGAGGCTGGCATCAGTGCGCAAACGATCGCACGGGCCGTCGAGGCGGGGGAGATCGACCGTATCTCGCGCGGGGTTT
ATCAGAAGCGTGGCGTAGAGGTCGAAGAGAACCAGATCCTAGCTGAAGCCCTCATGCGTGCCCCCAAAGGCGTTATCGCT
TTGGTATCGGCGCTTGCCTTTCATGGACTGACCGACCAAATGCCACGGCGCGTTTGGATGGCGATCGGGACGAGCGATTG
GGCGCCCGTGCAGTCCTATCCCCCTCTGCGCATAGTCCGTTTCACGCAGCCTTACCTGCGACAAGGCATCGAGCATCATG
TCATCGCTGGACTCGACGTCCCGATCTATTCGATCCCGAAGACGCTTGCCGACCTCTTCCGAAACCCCAAACTCGTCGAT
CGGTCAGTCGCTGTAGAAGGCCTGCGAGCAGCTCTTCAGCAACGCAAGGCCACTCCGAGCGCAATCGCCCACGCCGCGAA
GGCCGGCGGTGCTTGGAAGGTGATGCAACCCTATCTGGAGGCGCTCACCTCCAATGGCTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684