Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4207652..4208270 Replicon chromosome
Accession NZ_CP084207
Organism Escherichia coli strain EC2648-R1-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LDB15_RS20360 Protein ID WP_001291435.1
Coordinates 4208052..4208270 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LDB15_RS20355 Protein ID WP_000344800.1
Coordinates 4207652..4208026 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB15_RS20345 (4202741) 4202741..4203934 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LDB15_RS20350 (4203957) 4203957..4207106 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LDB15_RS20355 (4207652) 4207652..4208026 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LDB15_RS20360 (4208052) 4208052..4208270 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LDB15_RS20365 (4208442) 4208442..4208993 + 552 WP_000102564.1 maltose O-acetyltransferase -
LDB15_RS20370 (4209109) 4209109..4209579 + 471 WP_001716237.1 YlaC family protein -
LDB15_RS20375 (4209743) 4209743..4211293 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LDB15_RS20380 (4211335) 4211335..4211688 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LDB15_RS20390 (4212067) 4212067..4212378 + 312 WP_000409911.1 MGMT family protein -
LDB15_RS20395 (4212409) 4212409..4212981 - 573 WP_000779842.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T218258 WP_001291435.1 NZ_CP084207:4208052-4208270 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T218258 NZ_CP115713:c2207253-2206843 [Rhodopseudomonas palustris]
ATGAGTGGCTGGATGCTCGATACCAACGTCGCCAGCCATGTCATCAGGGGCGACCGGCGCGAGATCATCGAGCGGCTAGT
CGCTTTGCCGATCTCGGACGTCGTCATCTCATCGATCACAGAAGGTGAACTCCTCTACGGCCTGGCTAAGCGCGGCTATC
CCACCGCGTTATCCGAACGCGTCCGAGAATTTCTACTCCGCGTCGACGTCCTACCGTGGGATCACGAGGTGACGAAGACT
TACGCCGACCTGCGCGCAGTCTGCGAAGCCAAAGGCGTCACACTCTCTCCTCTCGACATGATGATCGCTGCCCATGCCGC
TGCGACAAACGCAACGTTGGTCACGCGCGACAAGGCCTTCAGCCGCGTGCCTTCACCATTGCGGATCGCGGACTGGGCAG
AGATGAGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT218258 WP_000344800.1 NZ_CP084207:4207652-4208026 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT218258 NZ_CP115713:c2207513-2207250 [Rhodopseudomonas palustris]
GTGACGTCTCGAACCGCCAAGATTTTCACAACTGGACGCAGCCAAGCTGTCCGACTGCCAGCGGAGTTTCGCTTCGAGGG
CAGCGAGGTGTTTGTACGCCGTGATCCGCGGACCGGCGATGTCATTCTGTCCCGAAAACCGGACTCCTGGGACGGCCTAC
TCCAGCTTCACCAGTCTGCGGATGTACCCGGCGACTTCTTGGGACCGTCCGACCGTAGCCAGCTTCCGCAGGATCGCGAT
CCGTTCGAAGGCTGGACCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References