Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3178677..3179315 Replicon chromosome
Accession NZ_CP084207
Organism Escherichia coli strain EC2648-R1-1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LDB15_RS15290 Protein ID WP_000813794.1
Coordinates 3179139..3179315 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LDB15_RS15285 Protein ID WP_001270286.1
Coordinates 3178677..3179093 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB15_RS15265 (3173829) 3173829..3174770 - 942 WP_001251304.1 ABC transporter permease -
LDB15_RS15270 (3174771) 3174771..3175784 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
LDB15_RS15275 (3175802) 3175802..3176947 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
LDB15_RS15280 (3177192) 3177192..3178598 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
LDB15_RS15285 (3178677) 3178677..3179093 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LDB15_RS15290 (3179139) 3179139..3179315 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LDB15_RS15295 (3179537) 3179537..3179767 + 231 WP_000494244.1 YncJ family protein -
LDB15_RS15300 (3179859) 3179859..3181820 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LDB15_RS15305 (3181893) 3181893..3182429 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
LDB15_RS15310 (3182521) 3182521..3183696 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T218257 WP_000813794.1 NZ_CP084207:c3179315-3179139 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T218257 NZ_CP115713:c1751036-1750740 [Rhodopseudomonas palustris]
ATGATTGACGTTCGCGAAACAATGGATTTCACGAACTGGCTCGCCGCCTTGAGCGACCTGCGCGCCCGTCTGCAGATCGT
TCGCCGGATCGACCGGATTGCCGCGGGAAACTTCGGTGATGCTAAATCGGTTGGTGGCGCGGTGAAGGAGCTTCGGATCG
ACTACGGACCGGGCTACCGAGTTTACTACACGCGGCGCGGCGACACTGTCGTTATCCTGCTCTGCGGCGGCGACAAGCGG
ACGCAAAGCAAGGACATCCGGAAGGCCAAGGAAATTGCAGAGGCGTTGGAGGACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT218257 WP_001270286.1 NZ_CP084207:c3179093-3178677 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT218257 NZ_CP115713:c1750737-1750420 [Rhodopseudomonas palustris]
ATGAAGGTCAGTAAACTGAAGAAGTTCAACGCGGCCGAACACCTTCGTACACCGGAAGCACGCGCGGAGTATCTGAACAT
CGTTCTCGCCGACGGCGACCCCAACGAAGTGCGTGACGCACTCAGTCTCGTCGCTCGCGCGCAGGGCATGAGCGCGATCG
CCAAGGCCGCTGGCGTCACCAGAGAGGGACTCTACAAGACGCTTGGTGAGAACGGTAACCCCGAGTTCGCAACCATCCTC
CGGGTGTTCGGCGCCATGGGATTCCGGTTGACGGCAGAACTGGCCAAAACCAAGCCGAGAAGGAAAGCCGTGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References