Detailed information of TA system TA218246

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2611434..2612265
• Replicon: chromosome
• Accession: NZ_CP084207
• Organism: Escherichia coli strain EC2648-R1-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: LDB15_RS12400
• Protein ID: WP_000854814.1
• Coordinates: 2611434..2611808 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: LDB15_RS12405
• Protein ID: WP_001285584.1
• Coordinates: 2611897..2612265 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB15_RS12360 (2606830)	2606830..2607996	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
LDB15_RS12365 (2608115)	2608115..2608588	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
LDB15_RS12370 (2608786)	2608786..2609844	+	1059	WP_001200891.1	FUSC family protein	-
LDB15_RS12375 (2610016)	2610016..2610345	+	330	WP_000450409.1	DUF496 family protein	-
LDB15_RS12380 (2610446)	2610446..2610580	-	135	WP_001297944.1	EutP/PduV family microcompartment system protein	-
LDB15_RS12385 (2610700)	2610700..2610828	+	129	Protein_2415	transposase domain-containing protein	-
LDB15_RS12390 (2611117)	2611117..2611197	-	81	Protein_2416	hypothetical protein	-
LDB15_RS12395 (2611243)	2611243..2611437	-	195	WP_000988600.1	DUF5983 family protein	-
LDB15_RS12400 (2611434)	2611434..2611808	-	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
LDB15_RS12405 (2611897)	2611897..2612265	-	369	WP_001285584.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
LDB15_RS12410 (2612339)	2612339..2612560	-	222	WP_000692323.1	DUF987 domain-containing protein	-
LDB15_RS12415 (2612623)	2612623..2613069	-	447	WP_000187523.1	RadC family protein	-
LDB15_RS12420 (2613066)	2613066..2614598	-	1533	WP_001350525.1	protein YeeR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T218246 WP_000854814.1 NZ_CP084207:c2611808-2611434 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T218246 NZ_CP115712:321320-321688 [Kosakonia pseudosacchari]
ATGACGATTCAGTTTATCTCAGCGGAAGAGATTGTTCGCTTTCACGATAGGTTGCTGCAGGCCACTCCCGGCGTCGCGGG
GATGGCAGATCCAGGACGTGCGGAAGCGTTACTCTATCGCGTGCTTAACCGCTATGAATATGAAGGCATAAACGATATCT
GGATGCTGGCGGCGATGCATCTTTTGGCCATCGCGCGCGGTCATATATTCAATGACGGCAACAAACGCACCGCATTATTT
ATTACCTTACTGTTTCTGCGACGTAATGGTATTGAACTTGCCTCAAACCCCGCTTTTGTCGATTTAACCGTGCGCGCGGC
GGCAGGTCAGCTTTCGCTTGAAGAGATCGCCAATCAGTTGCGCGGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT218246 WP_001285584.1 NZ_CP084207:c2612265-2611897 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT218246 NZ_CP115712:321102-321323 [Kosakonia pseudosacchari]
ATGCAAACCATGAATTATAGCGAAGCCCGCCAAAACCTGGCCGCAGCGCTGGACGTTGCAGTCAGTGGCACGCCCGTTAC
CATCACACGTCGGGGACATCAAGCCGCGGTTATTATCAGCGCAGAAGAGTTCGAACGTTATCAAAAAGCCAAAATGGATG
ACGAATTCGCCGCCATTATGGCGGTTCACGGAAATGAAATTAGAGAACTTGCGGACAAATGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6