Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1653839..1654493 Replicon chromosome
Accession NZ_CP084207
Organism Escherichia coli strain EC2648-R1-1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LDB15_RS07925 Protein ID WP_000244777.1
Coordinates 1654086..1654493 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LDB15_RS07920 Protein ID WP_000354046.1
Coordinates 1653839..1654105 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB15_RS07895 (1649008) 1649008..1649751 + 744 WP_000951964.1 SDR family oxidoreductase -
LDB15_RS07900 (1649808) 1649808..1651241 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LDB15_RS07905 (1651286) 1651286..1651597 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LDB15_RS07910 (1651761) 1651761..1652420 + 660 WP_072647361.1 hemolysin III family protein -
LDB15_RS07915 (1652616) 1652616..1653596 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LDB15_RS07920 (1653839) 1653839..1654105 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LDB15_RS07925 (1654086) 1654086..1654493 + 408 WP_000244777.1 protein YgfX Toxin
LDB15_RS07930 (1654533) 1654533..1655054 - 522 WP_001055874.1 flavodoxin FldB -
LDB15_RS07935 (1655166) 1655166..1656062 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LDB15_RS07940 (1656087) 1656087..1656797 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LDB15_RS07945 (1656803) 1656803..1658536 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T218241 WP_000244777.1 NZ_CP084207:1654086-1654493 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T218241 NZ_CP115708:c110262-109780 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT218241 WP_000354046.1 NZ_CP084207:1653839-1654105 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT218241 NZ_CP115708:c110531-110253 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References