Detailed information of TA system TA218223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3948659..3949473
• Replicon: chromosome
• Accession: NZ_CP084205
• Organism: Escherichia coli strain EC2648-R1

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: LDB14_RS19240
• Protein ID: WP_001054376.1
• Coordinates: 3948659..3948916 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: LDB14_RS19245
• Protein ID: WP_001309181.1
• Coordinates: 3948928..3949473 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB14_RS19215 (3943947)	3943947..3945053	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
LDB14_RS19220 (3945118)	3945118..3946098	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
LDB14_RS19225 (3946208)	3946208..3946413	+	206	Protein_3777	HNH endonuclease	-
LDB14_RS19230 (3946681)	3946681..3947921	-	1241	Protein_3778	helicase YjhR	-
LDB14_RS19235 (3948037)	3948037..3948168	+	132	WP_001309182.1	hypothetical protein	-
LDB14_RS19240 (3948659)	3948659..3948916	+	258	WP_001054376.1	YjhX family toxin	Toxin
LDB14_RS19245 (3948928)	3948928..3949473	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
LDB14_RS19250 (3949529)	3949529..3950275	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
LDB14_RS19255 (3950444)	3950444..3950662	+	219	Protein_3783	hypothetical protein	-
LDB14_RS19260 (3950700)	3950700..3950816	+	117	Protein_3784	VOC family protein	-
LDB14_RS19265 (3951061)	3951061..3952182	+	1122	WP_000010829.1	M42 family metallopeptidase	-
LDB14_RS19270 (3952179)	3952179..3952457	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
LDB14_RS19275 (3952469)	3952469..3953782	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	3937669..3957697	20028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T218223 WP_001054376.1 NZ_CP084205:3948659-3948916 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T218223 NZ_CP115701:2019783-2019885 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT218223 WP_001309181.1 NZ_CP084205:3948928-3949473 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT218223 NZ_CP115701:c2019892-2019747 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8