Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3397561..3398179 Replicon chromosome
Accession NZ_CP084205
Organism Escherichia coli strain EC2648-R1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LDB14_RS16535 Protein ID WP_001291435.1
Coordinates 3397961..3398179 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LDB14_RS16530 Protein ID WP_000344800.1
Coordinates 3397561..3397935 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB14_RS16520 (3392650) 3392650..3393843 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LDB14_RS16525 (3393866) 3393866..3397015 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LDB14_RS16530 (3397561) 3397561..3397935 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LDB14_RS16535 (3397961) 3397961..3398179 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LDB14_RS16540 (3398351) 3398351..3398902 + 552 WP_000102564.1 maltose O-acetyltransferase -
LDB14_RS16545 (3399018) 3399018..3399488 + 471 WP_001716237.1 YlaC family protein -
LDB14_RS16550 (3399652) 3399652..3401202 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LDB14_RS16555 (3401244) 3401244..3401597 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LDB14_RS16565 (3401976) 3401976..3402287 + 312 WP_000409911.1 MGMT family protein -
LDB14_RS16570 (3402318) 3402318..3402890 - 573 WP_000779842.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T218217 WP_001291435.1 NZ_CP084205:3397961-3398179 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T218217 NZ_CP115697:c26959-26543 [Enterobacter hormaechei]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGATGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGAT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCACTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCATGCCATCGCCGCCGGCGCGGTACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT218217 WP_000344800.1 NZ_CP084205:3397561-3397935 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT218217 NZ_CP115697:c27186-26956 [Enterobacter hormaechei]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCGCGCAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGGGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References