Detailed information of TA system TA218193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 67293..67828
• Replicon: plasmid pCB1B
• Accession: NZ_CP084199
• Organism: Pantoea agglomerans strain CB1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8X8DPA1
• Locus tag: LDO72_RS22385
• Protein ID: WP_010257115.1
• Coordinates: 67541..67828 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LDO72_RS22380
• Protein ID: WP_039387444.1
• Coordinates: 67293..67544 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO72_RS22365 (LDO72_22385)	62643..64532	+	1890	WP_109653735.1	sigma-54-dependent Fis family transcriptional regulator	-
LDO72_RS22370 (LDO72_22390)	64628..65578	+	951	WP_275103373.1	sulfonate ABC transporter substrate-binding protein	-
LDO72_RS22375 (LDO72_22395)	65871..67004	+	1134	WP_235765258.1	glutathione-independent formaldehyde dehydrogenase	-
LDO72_RS22380 (LDO72_22400)	67293..67544	+	252	WP_039387444.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LDO72_RS22385 (LDO72_22405)	67541..67828	+	288	WP_010257115.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDO72_RS22390 (LDO72_22410)	68192..68506	+	315	WP_143790132.1	hypothetical protein	-
LDO72_RS22395 (LDO72_22415)	68503..69321	-	819	WP_275103374.1	DUF4225 domain-containing protein	-
LDO72_RS22400 (LDO72_22420)	69578..69808	+	231	Protein_62	helix-turn-helix domain-containing protein	-
LDO72_RS22405 (LDO72_22425)	69828..70112	+	285	Protein_63	IS3 family transposase	-
LDO72_RS22410 (LDO72_22430)	70209..71129	-	921	WP_069027204.1	Rpn family recombination-promoting nuclease/putative transposase	-
LDO72_RS22415 (LDO72_22435)	71538..71704	+	167	Protein_65	type II toxin-antitoxin system RelE/ParE family toxin	-
LDO72_RS22420 (LDO72_22440)	71986..72681	+	696	WP_143790070.1	haloacid dehalogenase type II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..171886	171886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11173.08 Da
• Isoelectric Point: 10.3856

>T218193 WP_010257115.1 NZ_CP084199:67541-67828 [Pantoea agglomerans]
VSYTVKFREEAFKEWQKLDKSLQQQFAKKLKKCCDNPHIPSARLRGIKDCYKIKLRASGFRLVYQVIDDQLVIAVVAVGK
RERSDVYHLASERMR

Nucleotide

• Length: 288 bp

>T218193 NZ_CP115672:c4658598-4658227 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGAGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9108.38 Da
• Isoelectric Point: 3.9507

>AT218193 WP_039387444.1 NZ_CP084199:67293-67544 [Pantoea agglomerans]
MPNIILSDTSASVSELKKNPMATVNAGDGYPVAILNRNQPAFYCVPAELYEQMLDALDDQELVKLVSERSGQRLLDVDLD
ALL

Nucleotide

• Length: 252 bp

>AT218193 NZ_CP115672:c4658843-4658601 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA