Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3164808..3165425 Replicon chromosome
Accession NZ_CP084197
Organism Pantoea agglomerans strain CB1

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag LDO72_RS15005 Protein ID WP_003850458.1
Coordinates 3165210..3165425 (+) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E0LTU8
Locus tag LDO72_RS15000 Protein ID WP_003850455.1
Coordinates 3164808..3165185 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO72_RS14970 (LDO72_14975) 3161115..3161369 + 255 WP_010671219.1 type B 50S ribosomal protein L31 -
LDO72_RS14975 (LDO72_14980) 3161381..3161521 + 141 WP_010255896.1 type B 50S ribosomal protein L36 -
LDO72_RS14980 (LDO72_14985) 3161567..3162445 - 879 WP_250863483.1 metal ABC transporter substrate-binding protein -
LDO72_RS14985 (LDO72_14990) 3162461..3163300 - 840 WP_010255890.1 metal ABC transporter permease -
LDO72_RS14990 (LDO72_14995) 3163297..3163965 - 669 WP_069026576.1 ABC transporter ATP-binding protein -
LDO72_RS14995 (LDO72_15000) 3164307..3164660 + 354 WP_275102480.1 hypothetical protein -
LDO72_RS15000 (LDO72_15005) 3164808..3165185 + 378 WP_003850455.1 Hha toxicity modulator TomB Antitoxin
LDO72_RS15005 (LDO72_15010) 3165210..3165425 + 216 WP_003850458.1 HHA domain-containing protein Toxin
LDO72_RS15015 (LDO72_15020) 3165832..3166143 + 312 WP_031592174.1 MGMT family protein -
LDO72_RS15020 (LDO72_15025) 3166183..3166740 - 558 WP_010255881.1 YbaY family lipoprotein -
LDO72_RS15025 (LDO72_15030) 3166931..3167794 + 864 WP_010255880.1 acyl-CoA thioesterase II -
LDO72_RS15030 (LDO72_15035) 3167845..3169131 - 1287 WP_010255877.1 ammonium transporter AmtB -
LDO72_RS15035 (LDO72_15040) 3169166..3169504 - 339 WP_003850469.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T218187 WP_003850458.1 NZ_CP084197:3165210-3165425 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T218187 NZ_CP115672:c303531-303229 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATAGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14636.25 Da        Isoelectric Point: 4.3976

>AT218187 WP_003850455.1 NZ_CP084197:3164808-3165185 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT218187 NZ_CP115672:c303222-302935 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A1I5AG55

References