Detailed information of TA system TA021817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 2227005..2227524
• Replicon: chromosome
• Accession: NC_008702
• Organism: Azoarcus sp. BH72

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A1K736
• Locus tag: AZO_RS10220
• Protein ID: WP_011765755.1
• Coordinates: 2227270..2227524 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A1K735
• Locus tag: AZO_RS10215
• Protein ID: WP_011765754.1
• Coordinates: 2227005..2227280 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZO_RS10190	2222358..2222840	-	483	WP_011765749.1	Fe-S cluster assembly transcriptional regulator IscR	-
AZO_RS10195	2222961..2223716	-	756	WP_011765750.1	serine O-acetyltransferase	-
AZO_RS10200	2223803..2224531	-	729	WP_011765751.1	RNA methyltransferase	-
AZO_RS10205	2224621..2225433	+	813	WP_011765752.1	inositol monophosphatase	-
AZO_RS10210	2225579..2226112	-	534	WP_011765753.1	SRPBCC family protein	-
AZO_RS10215	2227005..2227280	+	276	WP_011765754.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
AZO_RS10220	2227270..2227524	+	255	WP_011765755.1	Txe/YoeB family addiction module toxin	Toxin
AZO_RS10225	2227858..2228355	-	498	WP_011765756.1	GNAT family N-acetyltransferase	-
AZO_RS10230	2228352..2228645	-	294	WP_011765757.1	DUF1778 domain-containing protein	-
AZO_RS10235	2229090..2229269	+	180	WP_011765758.1	transposase	-
AZO_RS10240	2229309..2229731	+	423	WP_011765759.1	hypothetical protein	-
AZO_RS10245	2229728..2230090	+	363	WP_041642522.1	IS66 family insertion sequence element accessory protein TnpB	-
AZO_RS20195	2230123..2230368	+	246	WP_050976121.1	hypothetical protein	-
AZO_RS10250	2230417..2231724	+	1308	WP_158309402.1	IS66 family transposase	-
AZO_RS10255	2231721..2232221	+	501	WP_011765763.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10112.55 Da
• Isoelectric Point: 7.9191

>T21817 WP_011765755.1 NC_008702:2227270-2227524 [Azoarcus olearius]
MPIKFADHAWEDYLYWQQTDKAIVKRINQLIRDIQRSPFEGIGKPEPLRHHLSGYWSRRINDEHRLVYAVEDGCLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T21817 NC_008702:2227270-2227524 [Azoarcus olearius]
ATGCCGATTAAGTTCGCCGACCACGCCTGGGAGGACTATCTCTACTGGCAGCAGACCGACAAGGCGATCGTCAAGCGCAT
TAACCAGCTGATCCGGGACATCCAGCGCTCGCCCTTCGAGGGCATCGGCAAACCGGAGCCATTGCGACACCACCTCTCGG
GATACTGGTCCCGGCGCATCAACGACGAGCACCGCCTCGTCTACGCGGTGGAAGATGGCTGCCTCCTGATCGCCGCCTGT
CGCTATCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10210.30 Da
• Isoelectric Point: 4.3646

>AT21817 WP_011765754.1 NC_008702:2227005-2227280 [Azoarcus olearius]
MDALTYSYTRQHLTAVMDQVNEDRAPVLVTRQNGKPVVMMSLDDYNALEETAYLLRSPENARRLAASIEQLRRGGGQARE
LDEGNETPDAD

Nucleotide

• Length: 276 bp

>AT21817 NC_008702:2227005-2227280 [Azoarcus olearius]
ATGGACGCGCTGACTTACAGCTATACCCGACAGCATCTGACCGCGGTCATGGATCAGGTGAACGAAGACCGCGCCCCCGT
GCTGGTGACGCGCCAGAACGGGAAGCCCGTCGTGATGATGTCGCTCGACGACTACAACGCGCTCGAAGAGACGGCTTACC
TCCTGCGCAGCCCGGAAAATGCGCGTCGCCTCGCCGCCTCGATCGAGCAACTGCGCCGGGGCGGCGGCCAGGCCCGGGAA
CTGGATGAAGGCAACGAGACGCCCGATGCCGATTAA

Structures

Toxin

• AlphaFold DB: A1K736

Antitoxin

• AlphaFold DB: A1K735