Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2936597..2937407 Replicon chromosome
Accession NZ_CP084168
Organism Klebsiella pneumoniae strain B17602

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LDH50_RS14155 Protein ID WP_004178461.1
Coordinates 2936874..2937407 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LDH50_RS14150 Protein ID WP_002887278.1
Coordinates 2936597..2936863 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDH50_RS14130 (LDH50_14130) 2932469..2932813 - 345 WP_134920081.1 cation efflux system protein CusF -
LDH50_RS14135 (LDH50_14135) 2932831..2934216 - 1386 WP_004894567.1 efflux transporter outer membrane subunit -
LDH50_RS14140 (LDH50_14140) 2934388..2935071 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
LDH50_RS14145 (LDH50_14145) 2935061..2936494 + 1434 WP_004894565.1 Cu(+)/Ag(+) sensor histidine kinase -
LDH50_RS14150 (LDH50_14150) 2936597..2936863 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LDH50_RS14155 (LDH50_14155) 2936874..2937407 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LDH50_RS14160 (LDH50_14160) 2937455..2938576 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T218148 WP_004178461.1 NZ_CP084168:2936874-2937407 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T218148 NZ_CP115659:2129881-2129983 [Kosakonia oryzendophytica]
GCCAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTTTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT218148 WP_002887278.1 NZ_CP084168:2936597-2936863 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT218148 NZ_CP115659:c2129983-2129821 [Kosakonia oryzendophytica]
AAAAAAAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TAATGCAGGCTAAGTCGCCTGGCACTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTCCGCGACAAAAGTGGTCAAAA
AAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References