Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2339799..2340424 Replicon chromosome
Accession NZ_CP084168
Organism Klebsiella pneumoniae strain B17602

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LDH50_RS11335 Protein ID WP_002882817.1
Coordinates 2340041..2340424 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LDH50_RS11330 Protein ID WP_004150355.1
Coordinates 2339799..2340041 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDH50_RS11305 (LDH50_11305) 2335790..2336389 + 600 WP_004151865.1 glucose-1-phosphatase -
LDH50_RS11310 (LDH50_11310) 2336383..2337243 + 861 WP_004146232.1 virulence factor BrkB family protein -
LDH50_RS11315 (LDH50_11315) 2337240..2337677 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LDH50_RS11320 (LDH50_11320) 2337722..2338663 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LDH50_RS11325 (LDH50_11325) 2338677..2339594 - 918 WP_004895173.1 alpha/beta hydrolase -
LDH50_RS11330 (LDH50_11330) 2339799..2340041 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LDH50_RS11335 (LDH50_11335) 2340041..2340424 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LDH50_RS11340 (LDH50_11340) 2340598..2341527 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LDH50_RS11345 (LDH50_11345) 2341524..2342159 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LDH50_RS11350 (LDH50_11350) 2342156..2343058 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T218146 WP_002882817.1 NZ_CP084168:2340041-2340424 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T218146 NZ_CP115659:74777-75181 [Kosakonia oryzendophytica]
ATGCTCAAATTTATGCTTGATACAAACATTTGTATTTTCACTATAAAAAATAAACCCGAAACTGTCCGCCGGGCATTCAA
TCAGCGTGACGGACAGATGTGTATCAGCTCTATCACATTGATGGAGCTAATTTATGGTGCAGAGAAATCGGCCGCGCCTG
AAAAAAACTTGCGTGTCGTTGAAGGGTTTACCGCCAGGCTGGAGGTGCTGCCTTATGGTATCGATGCGTCAGTGCATACT
GGTCAACTCAGGGCGGAGCTGGCAAGAGAAGGGGCACCCATCGGGCCTTATGATGCCATGATTGGTGCTCATGCCCGTTC
CTTGGGATTAATTCTCGTGACCAACAACACCCGCGAGTTTGTCCGAATTCCAGGTCTACGTTTGCAGGACTGGACGATGC
ATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT218146 WP_004150355.1 NZ_CP084168:2339799-2340041 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT218146 NZ_CP115659:74547-74777 [Kosakonia oryzendophytica]
ATGGAGAAAACGACACTTTTTAAAAGCAATCGCAGTCAGGCTGTACGTTTGCCAAAAGCCGTGGCTCTGCCAGAAGACGT
CAGGCACGTTGATATTATTGCTGTGGGGCGCACACGAATTATCACTCCATCAGGAGAAAGCTGGGATAGCTGGTTTGATG
GGGAGAATGCATCTGCTGATTTTATGACCGCGCGTGAGCAACCAGGGGATCAGGACAGGGAAGATTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References