Detailed information of TA system TA021813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1387808..1388443
• Replicon: chromosome
• Accession: NC_008698
• Organism: Thermofilum pendens Hrk 5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A1S053
• Locus tag: TPEN_RS07355
• Protein ID: WP_011753098.1
• Coordinates: 1388042..1388443 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: TPEN_RS07350
• Protein ID: WP_052885284.1
• Coordinates: 1387808..1388035 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TPEN_RS07315 (Tpen_1429)	1382909..1383445	+	537	WP_052885281.1	DUF3782 domain-containing protein	-
TPEN_RS07320 (Tpen_1430)	1383569..1384036	+	468	WP_245534158.1	peroxiredoxin	-
TPEN_RS07325 (Tpen_1431)	1384033..1385412	-	1380	WP_052885282.1	CehA/McbA family metallohydrolase	-
TPEN_RS07330 (Tpen_1432)	1385899..1386231	+	333	WP_281054513.1	HEPN domain-containing protein	-
TPEN_RS07335 (Tpen_1433)	1386206..1386607	+	402	WP_011753095.1	nucleotidyltransferase domain-containing protein	-
TPEN_RS07340 (Tpen_1434)	1386803..1387180	-	378	WP_052885283.1	HEPN domain-containing protein	-
TPEN_RS07345	1387210..1387701	-	492	WP_245534159.1	nucleotidyltransferase domain-containing protein	-
TPEN_RS07350 (Tpen_1435)	1387808..1388035	+	228	WP_052885284.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
TPEN_RS07355 (Tpen_1436)	1388042..1388443	+	402	WP_011753098.1	type II toxin-antitoxin system VapC family toxin	Toxin
TPEN_RS07360 (Tpen_1437)	1388453..1388998	-	546	WP_011753099.1	hypothetical protein	-
TPEN_RS07365 (Tpen_1438)	1389068..1389850	-	783	WP_187146324.1	hypothetical protein	-
TPEN_RS07370	1389802..1390254	-	453	WP_052885286.1	hypothetical protein	-
TPEN_RS07375 (Tpen_1439)	1390399..1391094	+	696	WP_052885287.1	hypothetical protein	-
TPEN_RS07380 (Tpen_1440)	1391091..1392710	-	1620	WP_011753102.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (31-121)

Antitoxin

• Post-segregation antitoxin CcdA (10-53)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14719.13 Da
• Isoelectric Point: 4.9477

>T21813 WP_011753098.1 NC_008698:1388042-1388443 [Thermofilum pendens Hrk 5]
MERRFLLDASSVVQALKLGNLRVIYGNYIQWLTVYEVVNALWKEALLAGSISVEEASNLVEVFAETVKLVEVLSPHPYEA
EILETAAKLGLTAYDASYVVLAIRNGLSLVTEDRVLREKAKGVVKAVSLREVL

Nucleotide

• Length: 402 bp

>T21813 NC_008698:1388042-1388443 [Thermofilum pendens Hrk 5]
ATGGAGAGGAGGTTCCTCCTGGACGCCTCGTCGGTCGTGCAGGCCTTGAAGCTCGGGAACTTGAGGGTAATCTACGGGAA
CTACATCCAGTGGCTTACCGTATACGAGGTTGTTAACGCGCTTTGGAAGGAGGCCCTGCTGGCCGGTAGTATAAGCGTCG
AGGAGGCCTCCAACCTGGTCGAGGTGTTCGCCGAGACCGTTAAGCTCGTAGAGGTGCTGAGCCCCCACCCGTACGAGGCG
GAGATACTGGAGACAGCCGCCAAGCTGGGGTTAACGGCTTACGACGCGTCCTACGTGGTGCTAGCGATAAGGAACGGTTT
ATCCCTGGTGACCGAGGACAGGGTGCTGAGGGAGAAGGCGAAAGGCGTAGTTAAAGCTGTGAGCCTAAGGGAAGTACTCT
AG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8946.36 Da
• Isoelectric Point: 10.3297

>AT21813 WP_052885284.1 NC_008698:1387808-1388035 [Thermofilum pendens Hrk 5]
MGGYVTVSAKVRRELKEEAERLGIRISEVLRRALEEEVKRRKLEELEKRLEAMRGALERIDVERVVRGIREDRER

Nucleotide

• Length: 228 bp

>AT21813 NC_008698:1387808-1388035 [Thermofilum pendens Hrk 5]
ATGGGTGGCTACGTTACCGTCTCCGCTAAGGTTAGGAGGGAGCTTAAAGAGGAGGCGGAGAGGCTGGGTATCAGGATTTC
CGAGGTTCTTAGGCGCGCGTTGGAGGAGGAGGTTAAGCGGCGCAAGCTGGAGGAGCTCGAGAAGAGGCTCGAAGCCATGC
GCGGCGCGCTGGAGAGGATAGACGTCGAGAGGGTTGTGAGGGGGATTAGGGAGGATAGGGAGAGGTAG

Structures

Toxin

• AlphaFold DB: A1S053