Detailed information of TA system TA218124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2091307..2091836
• Replicon: chromosome
• Accession: NZ_CP084107
• Organism: Staphylococcus aureus strain CC49/ST49-MSSA

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LB316_RS10295
• Protein ID: WP_000621175.1
• Coordinates: 2091307..2091669 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LB316_RS10300
• Protein ID: WP_000948331.1
• Coordinates: 2091666..2091836 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB316_RS10275 (2088286)	2088286..2089056	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
LB316_RS10280 (2089031)	2089031..2089510	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
LB316_RS10285 (2089512)	2089512..2089838	-	327	WP_001052491.1	anti-sigma factor antagonist	-
LB316_RS10290 (2089957)	2089957..2090958	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
LB316_RS10295 (2091307)	2091307..2091669	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LB316_RS10300 (2091666)	2091666..2091836	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LB316_RS10305 (2091921)	2091921..2093069	-	1149	WP_001281148.1	alanine racemase	-
LB316_RS10310 (2093135)	2093135..2093494	-	360	WP_000581197.1	holo-ACP synthase	-
LB316_RS10315 (2093498)	2093498..2093989	-	492	WP_001205916.1	PH domain-containing protein	-
LB316_RS10320 (2093976)	2093976..2095559	-	1584	WP_001294642.1	PH domain-containing protein	-
LB316_RS10325 (2095552)	2095552..2096031	-	480	WP_001287083.1	hypothetical protein	-
LB316_RS10330 (2096240)	2096240..2096800	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T218124 WP_000621175.1 NZ_CP084107:c2091669-2091307 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T218124 NZ_CP115622:13198-13485 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGATATACTAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT218124 WP_000948331.1 NZ_CP084107:c2091836-2091666 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT218124 NZ_CP115622:13472-13786 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1