Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 131550..132202 Replicon plasmid pC11-215kb
Accession NZ_CP084104
Organism Klebsiella pneumoniae strain C11

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag K8M04_RS25835 Protein ID WP_017901321.1
Coordinates 131550..131975 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag K8M04_RS25840 Protein ID WP_001261275.1
Coordinates 131972..132202 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M04_RS25800 (K8M04_25730) 127259..127375 + 117 Protein_136 transposase -
K8M04_RS25805 (K8M04_25735) 127500..127670 - 171 Protein_137 LysR family transcriptional regulator -
K8M04_RS25810 127681..127908 + 228 Protein_138 IS3 family transposase -
K8M04_RS25815 (K8M04_25745) 128000..129556 - 1557 WP_025999314.1 sensor domain-containing diguanylate cyclase -
K8M04_RS25820 129743..129916 - 174 Protein_140 nuclease -
K8M04_RS25825 (K8M04_25750) 129969..130505 - 537 Protein_141 integrase core domain-containing protein -
K8M04_RS25830 (K8M04_25755) 130564..131532 - 969 WP_172540637.1 IS5 family transposase -
K8M04_RS25835 (K8M04_25760) 131550..131975 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
K8M04_RS25840 (K8M04_25765) 131972..132202 - 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K8M04_RS25845 (K8M04_25770) 132495..133615 + 1121 WP_087728521.1 IS3 family transposase -
K8M04_RS25850 (K8M04_25775) 133680..136256 - 2577 WP_017901322.1 nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..215108 215108
- inside IScluster/Tn - - 130564..133615 3051


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T218116 WP_017901321.1 NZ_CP084104:c131975-131550 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T218116 NZ_CP115551:c4311143-4310835 [Salmonella enterica subsp. enterica serovar Bispebjerg]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT218116 WP_001261275.1 NZ_CP084104:c132202-131972 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT218116 NZ_CP115551:c4311385-4311146 [Salmonella enterica subsp. enterica serovar Bispebjerg]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References