Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 53639..54549 Replicon plasmid pC11-215kb
Accession NZ_CP084104
Organism Klebsiella pneumoniae strain C11

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag K8M04_RS25410 Protein ID WP_004144067.1
Coordinates 54079..54549 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag K8M04_RS25405 Protein ID WP_023287184.1
Coordinates 53639..54082 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M04_RS25385 (K8M04_25320) 49055..49804 + 750 Protein_53 IS5 family transposase -
K8M04_RS25390 (K8M04_25325) 49833..50801 + 969 WP_074424072.1 IS5 family transposase -
K8M04_RS25395 (K8M04_25330) 50997..52343 + 1347 WP_225517490.1 ThiF family adenylyltransferase -
K8M04_RS25400 (K8M04_25335) 52396..53538 + 1143 Protein_56 translesion error-prone DNA polymerase V subunit UmuC -
K8M04_RS25405 (K8M04_25340) 53639..54082 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
K8M04_RS25410 (K8M04_25345) 54079..54549 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
K8M04_RS25415 (K8M04_25350) 54636..55631 + 996 WP_180334945.1 IS5 family transposase -
K8M04_RS25420 (K8M04_25355) 55633..56601 + 969 WP_072096747.1 IS5 family transposase -
K8M04_RS25425 (K8M04_25360) 56790..57050 - 261 WP_017901409.1 hypothetical protein -
K8M04_RS25430 (K8M04_25365) 57355..58323 + 969 WP_226328120.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..215108 215108
- inside IScluster/Tn - - 46911..58323 11412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T218114 WP_004144067.1 NZ_CP084104:54079-54549 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T218114 NZ_CP115551:3675514-3675732 [Salmonella enterica subsp. enterica serovar Bispebjerg]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT218114 WP_023287184.1 NZ_CP084104:53639-54082 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT218114 NZ_CP115551:3675112-3675486 [Salmonella enterica subsp. enterica serovar Bispebjerg]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References