Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5094034..5094659 Replicon chromosome
Accession NZ_CP084103
Organism Klebsiella pneumoniae strain C11

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K8M04_RS24755 Protein ID WP_002882817.1
Coordinates 5094034..5094417 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K8M04_RS24760 Protein ID WP_004150355.1
Coordinates 5094417..5094659 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M04_RS24740 (K8M04_24670) 5091400..5092302 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K8M04_RS24745 (K8M04_24675) 5092299..5092934 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K8M04_RS24750 (K8M04_24680) 5092931..5093860 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K8M04_RS24755 (K8M04_24685) 5094034..5094417 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K8M04_RS24760 (K8M04_24690) 5094417..5094659 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K8M04_RS24765 (K8M04_24695) 5094864..5095781 + 918 WP_040171413.1 alpha/beta hydrolase -
K8M04_RS24770 (K8M04_24700) 5095796..5096737 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K8M04_RS24775 (K8M04_24705) 5096782..5097219 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K8M04_RS24780 (K8M04_24710) 5097216..5098076 - 861 WP_040171219.1 virulence factor BrkB family protein -
K8M04_RS24785 (K8M04_24715) 5098070..5098669 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T218113 WP_002882817.1 NZ_CP084103:c5094417-5094034 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T218113 NZ_CP115551:2427957-2428241 [Salmonella enterica subsp. enterica serovar Bispebjerg]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTACGGGAACGGCTCGAAAACCCTCGGGTTCCTGCTTCGCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGGCACAGAAGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT218113 WP_004150355.1 NZ_CP084103:c5094659-5094417 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT218113 NZ_CP115551:2427719-2427967 [Salmonella enterica subsp. enterica serovar Bispebjerg]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References